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相关概念视频

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Thermal Measurement Techniques in Analytical Microfluidic Devices
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支持NFC的光热基微流体纸张分析装置用于检测葡萄糖.

Kawin Khachornsakkul1,2, Ruben Del-Rio-Ruiz1,2, Cihan Asci1,2

  • 1Department of Electrical and Computer Engineering, Tufts University, Medford, MA 02155, USA. Kawin.khachornsakkul@tufts.edu.

The Analyst
|June 5, 2024
PubMed
概括

这项研究介绍了一种无酶的葡萄糖传感器,使用光热微流体纸张分析装置 (PT-μPAD) 与金纳米粒子和智能手机读取. 这种新的方法为临床诊断提供了灵敏,选择性和负担得起的葡萄糖量化方法.

科学领域:

  • 分析化学 分析化学
  • 材料科学 材料科学 材料科学
  • 生物医学工程 生物医学工程

背景情况:

  • 精确的葡萄糖监测对于糖尿病管理至关重要.
  • 现有的方法通常依赖于酶或复杂的仪器仪表,限制了可访问性.
  • 需要具有成本效益,灵敏和选择性的葡萄糖检测方法.

研究的目的:

  • 开发一种无酶的光热微流体纸张分析装置 (PT-μPAD) 用于葡萄糖量化.
  • 将近场通信 (NFC) 技术集成到基于智能手机的阅读输出中.
  • 为了证明可访问和负担得起的点照顾葡萄糖传感器的潜力.

主要方法:

  • 使用金纳米粒子 (AuNPs) 作为纳米酶和光热基质.
  • 由葡萄糖催化生成的过氧化 (H2O2),用于蚀刻AuNPs.
  • 通过在LED照明下对AuNPs的等离子加热采用光热检测.
  • 将PT-μPAD与智能手机读取便携式NFC平台集成.

主要成果:

  • 实现了 5.020.0 μmol L−1 (R2 = 0.9967) 的线性检测范围和 25.0 nmol L−1.1. 的检测极限 (LOD).
  • 在没有其他物质干扰的情况下对葡萄糖表现出显著的选择性.

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  • 在人类样本中获得了高精度 (99.73102.66%的恢复) 和精度 (RSD ≤3.53%).
  • 与传统的μPAD相比,显示出更高的灵敏度.

    • 结论:

    • 开发的PT-μPAD提供了一种敏感,选择性和无酶的葡萄糖量化方法.
    • 与NFC和智能手机读数的集成使得可访问和负担得起的点的护理诊断.
    • 该平台有望用于检测其他生物标志物和推进个性化医疗保健.