简单和快速的流动细胞计量试验用于检测体液中的恶性上皮细胞

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Cytopathology : official journal of the British Society for Clinical Cytology

|June 5, 2024

概括

流式细胞计 (FCM) 提供了一种简单,具有成本效益的方法,用于检测体液中的恶性上皮细胞,补充传统细胞学并减少误诊.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
细胞病理学细胞病理学

背景情况:

对身体液体进行恶性瘤的形态分析具有局限性.
流细胞计 (FCM) 正在成为治疗非血液性恶性瘤的强大工具.

研究的目的:

标准化一种快速简单的FCM测试,用于检测体液中的恶性上皮细胞.
为了评估FCM的诊断性能与传统细胞学相比.

主要方法:

一项前性研究涉及121个体液样本,这些样本经过细胞学和FCM处理.
开发一种光标记的单克隆抗体面板,针对细胞表面标记物.
将FCM结果与细胞学/细胞阻断发现进行比较.

主要成果:

通过两种方法,FCM的诊断性能与细胞学相提并论,其中52例为阳性,60例为阴性.
观察到9个不一致的病例,6个细胞学+/FCM-和3个FCM+/细胞学-.
聚类上皮细胞粘附分子 (EpCAM) - 具有高分散的阳性事件是FCM诊断的关键指标.

结论:

开发的FCM试验简单,具有成本效益和灵敏性,没有观察者间的变化.
FCM作为常规诊断细胞学的一个有价值的辅助,可能减少误诊.
限制包括在低细胞样本或缺乏EpCAM表达的样本中潜在的假阴性.

关键词:

在 EpCAM 中使用 EpCAM.这种癌症是癌症癌症.细胞科学细胞学喷出喷出喷出喷出流动细胞计量是流动细胞计量的方法.

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