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通过RNAi介导的帕皮林基因的淘汰会影响尼拉帕尔瓦塔卢根斯的卵化

Chao Zhang^1,2, Jin-Yi Zhang^1,2, Ni Wang^1,2

¹State Key Laboratory of Rice Biology and Breeding; Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.

Pest management science

|June 5, 2024

概括

针对棕色植物 (BPH) 的乳头蛋白基因 (NlPpn) 干扰了卵子的发育,并降低了化能力. 这项研究确定了NlPpn作为基于RNA干扰 (RNAi) 的害虫控制策略的潜在目标,以对抗这种破坏性大米害虫.

科学领域:

昆虫学昆虫学是一门学科.
分子生物学分子生物学
虫害管理虫害管理虫害管理

背景情况:

棕色虫 (BPH) 是一种主要的水害虫,具有快速的抗农药性.
开发新的害虫控制方法,如RNA干扰 (RNAi),至关重要.
帕皮林是一种糖蛋白,对底层膜结构和发育至关重要.

研究的目的:

为了研究BPH.在巴皮林同源基因 (NlPpn) 的功能.
评估NlPpn作为控制BPH的潜在RNAi目标.

主要方法:

在BPH中识别和分析NLPpn的基因表达.
通过RNA干扰 (RNAi) 来抑制NlPpn的表达.
对受影响卵的转录和超结构分析.
在害虫控制中应用NlPpn-dsRNA纳米片.

主要成果:

在BPH卵中,NlPpn转录是丰富的.
向NlPpn的RNAi导致了不化卵和胚胎发育不良.
观察到与皮质结构相关的基因表达的显著变化.
超结构分析揭示了蛋的血清内膜缺陷.
局部应用NlPpn-dsRNA降低了卵子化能力.

结论:

对于BPH卵子发育和血清皮质完整性来说,NlPpn是必不可少的.
基于RNAi的BPH害虫管理中,NLPpn是一个有希望的目标,特别是在卵阶段.

关键词:

尼拉帕尔瓦塔 (尼拉帕尔瓦塔) 装饰品这是一种RNA干扰.胚胎发生是胚胎发生.纳米薄膜的使用方法帕皮林帕皮林是一个

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