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在克罗恩氏病中揭示微生物改变和宿主基因乱之间的联系通过TAHMC
Huijun Chang1, Yongshuai Liu1, Yue Wang1
1Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, 250012, China.
Advanced biology
|June 5, 2024
概括
一种新方法,宿主微生物组交叉的转录组分析 (TAHMC),将组织微生物与宿主基因表达联系起来. 这种方法有助于理解克罗恩病 (CD) 和识别诊断生物标志物.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 免疫学 免疫学 免疫学
背景情况:
- 在将组织微生物群落与宿主基因表达相关联的方法中存在差距.
- 了解宿主微生物群相互作用对于克罗恩病 (CD) 等疾病至关重要.
研究的目的:
- 引入一个新的计算管道,宿主-微生物组交叉的转录组分析 (TAHMC),用于分析宿主-微生物相互作用.
- 在CD中,确定组织微生物组与宿主免疫之间的关联.
- 为CD开发潜在的诊断生物标志物.
主要方法:
- 开发了TAHMC管道,以处理大量RNA-seq数据,同时进行宿主基因表达和微生物量化.
- 利用机器学习在宿主分子 (mRNA, lncRNA) 和微生物之间构建关联网络.
- 使用与Caco-2细胞共同培养的Faecalibacterium prausnitzii进行实验验证.
主要成果:
- TAHMC精确地从CD RNA-seq.q.中恢复宿主基因表达和微生物数据.
- 在CD中确定了组织微生物组与宿主免疫之间的关联.
- 发现了独特的宿主基因和微生物,对CD有潜在的诊断实用性.
- 在实验中验证了宿主基因表达的微生物调节.
结论:
- TAHMC管道提供了一种可靠的方法来研究组织中宿主微生物群交叉通话.
- 这些发现揭示了CD中的微生物失调和改变宿主基因表达之间的潜在因果关系.
- 这项研究为开发基于微生物组的CD诊断和治疗方法提供了基础.
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