橄叶提取物降低了SARS-CoV-2进入关键酶ACE-2,TMPRSS2和富林的蛋白质表达
Abdurrahim Kocyigit1,2, Ebru Kanımdan1,3, Vildan Betul Yenigun1,3
1Bezmialem Vakıf University, Faculty of Medicine, Department of Medical Biochemistry, Istanbul, Turkey.
Chemistry & biodiversity
|June 5, 2024
概括
橄叶提取物 (OLE) 通过减少关键的病毒进入蛋白质,显示出对抗SARS-CoV-2的希望. 这种天然化合物可能为COVID-19治疗提供新的治疗途径.
科学领域:
- 生物化学 生化学
- 病毒学 病毒学
- 药理学 药理学是指药理学的学科.
背景情况:
- SARS-CoV-2 突变挑战了疫苗的有效性,需要新的治疗方法.
- 针对病毒进入机制,包括ACE-2,TMPRSS2和Furin,对于COVID-19治疗至关重要.
- 橄叶提取物 (OLE) 显示出对抗COVID-19的潜力,但其机制需要进一步调查.
研究的目的:
- 调查橄叶提取物 (OLE) 对 ангиотензин转化酶-2 (ACE-2),跨膜蛋白酶血清亚型2 (TMPRSS2) 和富林的表达的影响.
- 评估OLE作为一种治疗药物对抗SARS-CoV-2入侵的潜力.
主要方法:
- 用细胞培养研究来评估OLE的影响.
- 光度法测量了总,黄酸盐含量和抗氧化能力.
- 液体LC-HR-MS量化了欧蛋白的水平. 液体LC-HR-MS量化了欧蛋白的水平.
- 西方血栓分析了ACE-2,TMPRSS2和Furin蛋白质的表达.
- 用ATP水平通过光度测量来评估细胞活力.
主要成果:
- 在剂量取决的方式上,OLE显著降低了ACE-2,TMPRSS2和Furin蛋白表达.
- 这些病毒进入蛋白质的最高抑制值在100μg/ml的OLE时观察到.
- OLE显示出抗氧化能力,并含有可测量的oleuropein.
结论:
- 橄叶提取物 (OLE) 通过抑制关键病毒进入蛋白质,显示出作为COVID-19治疗候选者的潜力.
- 为了验证这些发现,需要进一步研究涉及与病毒的同时感染.
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