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酵母β-葡萄糖通过dectin-1促进抗病毒I型干扰素反应
Jiaqi Wang1, Xuemin Jin2, Shihan Yan1
1Key Lab for Zoonoses Research, Ministry of Education, Animal Genome Editing Technology Innovation Center, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, China.
Veterinary microbiology
|June 5, 2024
概括
β-葡萄糖 (BG) 通过通过Dectin-1增强I型干扰素 (IFN) 来增强对伪狂犬病病毒 (PRV) 的训练免疫力. 口服BG显示了广泛的疫苗益处,这表明它可以作为料添加剂使用.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 兽医医学 兽医医学 兽医医学
背景情况:
- 伪狂热病毒 (PRV),一个alphaherpesvirus,构成了显著的动物性传染病威胁.
- 通过Dectin-1传感诱导β-葡萄糖 (BG) 的训练免疫提供了潜在的抗病毒策略.
- 在包括PRV在内的alphaherpesvirus感染中训练有素免疫的作用仍然在很大程度上未被探索.
研究的目的:
- 在受过训练的免疫环境中,研究β-葡萄糖 (BG) 对伪狂犬病病毒 (PRV) 的抗病毒作用.
- 阐明BG抗病毒特性背后的Dectin-1介导机制.
- 评估BG作为免疫调节剂的潜力,以提高猪的疫苗疗效.
主要方法:
- 预先用BG治疗小鼠,随后感染PRV,以评估存活率,体重减轻,组织学损伤和病毒载量.
- 测量了I型干扰素 (IFN) 的血清水平,并使用Dectin-1抗剂重复实验.
- 使用猪和小鼠巨细胞来确认Dectin-1介导的作用. 小猪接种了BG和PRV疫苗,随后对PRV特异性IgG,I型IFN和对其他疫苗的反应进行了评估.
主要成果:
- 在小鼠中,BG预治疗显著改善了生存率,减轻了体重减轻和组织损伤,并减少了PRV拷贝数.
- BG的使用显著增加了I型血清IFN水平,这种效应取决于Dectin-1信号传递.
- 在小猪中,BG增强了PRV特异性IgG和I型IFN水平,并大大改善了对其他疫苗 (PRRSV,CSF) 的反应,口服比注射更具成本效益和有效性.
结论:
- β-葡萄糖 (BG) 通过通过DECTIN-1介导的I型干扰素 (IFN) 的诱导,使人免受伪病毒 (PRV) 感染,从而建立训练免疫力.
- BG 显示出广泛的免疫调节作用,增强了各种疫苗在小猪中的疗效.
- 作为一种具有成本效益的料添加剂,BG具有巨大的潜力,用于控制PRV和其他牲畜中新出现的病毒感染.
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