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使用斑马鱼胚胎对二甲二甲酸的毒性评估:潜在的心脏毒性

Azza Naïja¹, Yoshifumi Horie², Sonia Boughattas¹

¹Biomedical Research Center, Qatar University, Doha, Qatar.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

|June 5, 2024

概括

双乙基甲酸盐 (DEHP) 是有毒的增塑剂. 这项研究表明,DEHP在斑马鱼胚胎中引起身体形和心脏毒性,即使度低.

科学领域:

环境毒理学环境毒理学
发育生物学是发展生物学.
心血管研究的心血管研究.

背景情况:

塑化剂,如二二乙烯基甲酸盐 (DEHP),是新出现的污染物.
众所周知,DEHP会对环境产生不利影响.
斑马鱼胚胎是评估化学毒性的敏感模型.

研究的目的:

为了调查DEHP在斑马鱼胚胎中的潜在毒性.
评估DEHP对胚胎发育和心脏功能的影响.
为了确定DEHP在不同度中的心脏毒性潜力.

主要方法:

斑马鱼胚胎在受精后96小时 (hpf) 暴露在DEHP的5个上升度 (0.008686 mg/L) 中.
胚胎毒性评估包括生存率,化率和形态形.
对心脏和炎症标志物的基因表达分析以及心脏功能分析进行了.

主要成果:

暴露DEHP导致预期化在48hpf和多个身体形.
在96 hpf时观察到tbx20,bcl2和il1b基因表达的显著变化.
DEHP显著影响心脏功能,影响动脉脉冲和后枢纽静脉 (PCV) 中的血液流动速度.

结论:

在斑马鱼胚胎中,DEHP表现出显著的毒性,诱导发育异常和心脏毒性.
即使是低度的DEHP也可能构成风险,突出其作为有毒环境污染物的潜力.
这些发现强调需要进一步调查DEHP的长期影响和环境影响.

关键词:

心脏毒性有关心脏的毒性.在DEHP中,DEHP是DEHP.基因表达基因表达心脏的功能心脏的功能斑马鱼胚胎斑马鱼的胚胎

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