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描述两种不同的基于酸盐的生物墨水,以及黑色素瘤生长在黑色素瘤生长中的影响
Raphael Schipka1, Stefanie Heltmann-Meyer1, Dominik Schneidereit2
1Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nürnberg, 91054, Erlangen, Germany.
Scientific reports
|June 5, 2024
概括
基于酸盐的两种生物墨水,酸盐-二甲凝 (ADA-GEL) 和酸盐/酸/凝 (Alg/HA/Gel),在3D生物打印中不同影响黑色素瘤细胞行为. ADA-GEL促进传播和迁移,而Alg/HA/Gel支持封装的殖民地形成.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 细胞生物学 细胞生物学
背景情况:
- 基于挤出的生物打印是一种关键的生物制造技术.
- 生物墨的特性极大地影响细胞行为,构建细胞的发育.
- 了解生物墨细胞相互作用对于开发有效的组织模型至关重要.
研究的目的:
- 为了比较两种不同的基于酸盐的生物墨水对黑色素瘤细胞行为的影响.
- 为了评估3D生物打印结构中的刚性,收缩,细胞活动和殖民地形成.
- 为了确定酸盐-二甲等 (ADA-GEL) 和酸盐/酸/等 (Alg/HA/Gel) 生物墨水对黑色素瘤细胞培养的适用性.
主要方法:
- 用于评估凝硬度的风湿学测量.
- 在ADA-GEL和Alg/HA/Gel生物墨水中3D生物打印Mel Im黑色素瘤细胞.
- 多光子显微镜用于观察细胞增殖,扩散和迁移.
- 扫描电子显微镜和组织学用于结构和降解分析.
主要成果:
- 这两种生物墨水都在15天内支持黑色素瘤细胞的增殖.
- ADA-GEL表现出显著的收缩,受细胞活动和共价交叉连接的影响.
- 在ADA-GEL中,细胞表现出繁殖,扩散和迁移,与Alg/HA/Gel中的球形殖民地不同.
- ADA-GEL显示了降解和多层生长,而Alg/HA/Gel显示了较少的脱离细胞和更深的殖民地亡.
结论:
- 生物墨的组成显著决定了黑色素瘤细胞的行为,包括扩散,迁移和殖民地形成.
- 与Alg/HA/Gel相比,ADA-GEL支持更动态的细胞相互作用和矩阵重塑.
- 生物墨水特性的表征对于优化癌症建模中的3D生物打印应用至关重要.
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