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超分子组合激活的单分子光共振能量转移用于近红外向细胞成像

Xiaolu Zhou¹, Xue Bai¹, Fangjian Shang¹

¹College of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin, P. R. China.

Nature communications

|June 5, 2024

概括

研究人员开发了一种新的单分子系统,用于光共振能量传输. 该系统表现出大量的斯托克斯转移和近红外辐射,使得向癌细胞成像成为可能.

科学领域:

超分子化学超分子化学
有机化学有机化学
生物医学成像技术生物医学成像技术

背景情况:

有机光共振能量转移 (PhRET) 是一个关键的研究领域.
开发具有理想光学特性的单分子PhRET系统仍然具有挑战性.

研究的目的:

构建一个单分子PhRET系统,具有很大的斯托克斯转移和近红外辐射.
研究超分子组合对PhRET特性的影响.
将开发的系统应用于癌细胞中的线粒体向成像.

主要方法:

合成一种基桥接的甲基-四乙烯-乙烯-甲衍生物.
使用cucurbit[n]urils (n=7,8) 和β-cyclodextrin修饰的氨酸构建超分子组件.
超分子结构和光学特性 (斯托克斯转移,辐射光谱) 的表征.
对癌细胞中线粒体向成像系统的评估.

主要成果:

一个单分子PhRET系统的367纳米斯托克斯转移和近红外辐射成功构建.
在540 nm的距离下,不同类型的库库比图里尔分子调节的超分子形态 (纳米颗粒,纳米棒,纳米板) 和光的固体比例变化.
三级联合组装和二级组装激活了分子内PhRET,在700nm产生近红外延迟光.
该系统在癌细胞中展示了有效的线粒体向成像.

结论:

超分子组装提供了一个强大的策略来调整有机PhRET系统的光学特性.
开发的近红外发射系统适用于先进的生物医学成像应用.
这项工作为为theranostics设计功能性超分子材料开辟了新的途径.

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