相关实验视频
Updated: Jun 24, 2025

15:53
Isolation and Biophysical Study of Fruit Cuticles
Published on: March 30, 2012
16.7K
在果实成熟过程中,低氧环境对番茄细胞壁组成的影响
Agata Leszczuk1, Nataliia Kutyrieva-Nowak2, Artur Nowak3
1Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, Lublin, 20-290, Poland. a.leszczuk@ipan.lublin.pl.
BMC plant biology
|June 5, 2024
概括
低氧气条件改变了番茄细胞壁的组成和成熟. 无氧 (0%氧气) 加快成熟,而低氧 (5%氧气) 减缓成熟,表明有保护性反应.
科学领域:
- 植物生理学 植物生理学
- 果实成熟的生物学
- 细胞壁生物化学 细胞壁生物化学
背景情况:
- 氧气度严重影响水果的保质期和质量.
- 了解细胞壁对低氧的反应对于水果储存至关重要.
- 番茄 (Solanum lycopersicum) 的成熟过程对氧气水平很敏感.
研究的目的:
- 识别受番茄低氧影响的细胞壁组件.
- 确定无氧 (0% O2) 和低氧 (5% O2) 条件对水果成熟的影响.
- 分析特定细胞壁多糖和蛋白质的变化.
主要方法:
- 在不同的成熟阶段对番茄果实进行比较分析.
- 在无氧 (0% O2) 和低氧 (5% O2) 条件下储存.
- 微观免疫标记,酶活性测定和细胞壁组件的分子概况.
主要成果:
- 低氧压力显著改变了细胞壁的组成,包括蛋白质含量和多糖分布.
- 观察到的变化包括焦糖沉积和酶活动的改变 (例如β-1,3-葡萄糖酶,GPX).
- 无氧条件加快了成熟变化,而低氧条件减缓了成熟变化,红色成熟的番茄的影响比破碎阶段番茄更明显.
结论:
- 低氧和无氧条件诱导番茄细胞壁中的分子变化.
- 这些变化代表了调节成熟的调节和保护机制.
- 细胞壁的组成是番茄在储存期间对氧气水平变化的反应的一个关键因素.
相关概念视频
Plant Cell Wall
56.5K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
56.5K
Radical Autoxidation
2.1K
The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
2.1K
Necrosis
4.4K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
4.4K
Tonicity in Plants
53.3K
Tonicity describes the capacity of a cell to lose or gain water. It depends on the quantity of solute that does not penetrate the membrane. Tonicity delimits the magnitude and direction of osmosis and results in three possible scenarios that alter the volume of a cell: hypertonicity, hypotonicity, and isotonicity. Due to differences in structure and physiology, tonicity of plant cells is different from that of animal cells in some scenarios.
53.3K
Role of Microtubules in Cell Wall Deposition
2.4K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
2.4K

