相关实验视频

Updated: Aug 18, 2026

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

通过通过Traf5-MAPK信号通路调节微质极化,CD36删除可以防止白质损伤

Xiaoxiang Hou¹, Xiaolin Qu², Wen Chen¹

¹Department of Neurosurgery, Changzheng Hospital, Naval Medical University, 415 Fengyang Rd, Shanghai, China.

Journal of neuroinflammation

|June 5, 2024

概括

抑制CD36通过通过Traf5-MAPK通路减少亲炎性微质偏振,从而减少创伤性脑损伤 (TBI) 后的白质损伤 (WMI). 这为TBI相关的神经炎症提供了潜在的治疗策略.

科学领域:

神经科学是一个神经科学.
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

创伤性脑损伤 (TBI) 经常导致白质损伤 (WMI),导致神经系统缺陷.
CD36受体和微质极化与神经系统疾病有关,但它们在TBI诱导的WMI中的具体作用尚不清楚.

研究的目的:

研究CD36在TBI后调节微质偏振和白质损伤中的作用.
阐明CD36在TBI中的作用的潜在分子机制.

主要方法:

已建立的小鼠TBI模型使用受控皮质冲击 (CCI).
评估了CD36表达 (qPCR,西部斑,免疫光) 和WMI (电子显微镜,LFB染色).
利用转录组测序和体外共同培养系统来探索分子通路和细胞相互作用.

主要成果:

创伤后CD36表达显著增加,特别是在微质细胞和星球细胞中.
CD36的遗传删除减少了TBI诱导的WMI和炎症性微质极化.
确定了Traf5-MAPK信号通路对于调解CD36对微质极化影响至关重要.

结论:

CD36抑制通过通过Traf5-MAPK通路抑制亲炎性微质偏振,减轻TBI诱导的WMI.
向CD36为治疗TBI后的神经炎症和WMI提供了潜在的治疗途径.

关键词:

CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 微质的极化是微质的极化创伤性脑损伤是一种创伤性脑损伤.白质损伤的伤害是白质的伤害.

更多相关视频

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Published on: April 13, 2017

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Published on: February 16, 2018

相关概念视频

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...