野生和栽培葡萄树之间的表观遗传差异凸显了在作物化过程中DNA甲基化的贡献
Alberto Rodriguez-Izquierdo1, David Carrasco1, Lakshay Anand2
1Centro de Biotecnología y Genómica de Plantas (CBGP-INIA), CSIC - Universidad Politécnica de Madrid, Campus Montegancedo, Madrid, Spain.
BMC plant biology
|June 5, 2024
概括
葡萄藤化涉及表观遗传变化,特别是DNA甲基化,影响特征. 种植的葡萄树表现出更高的甲基化,影响与压力和防御反应相关的基因.
科学领域:
- 植物遗传学 植物遗传学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 农业科学 农业科学
背景情况:
- 葡萄藤化固定了可取的特征,但表型变异仍然存在.
- 身体突变和表观遗传变异有助于葡萄藤表型.
- 提议在化过程中将表型变异性作为获得表型变异性的关键调节者.
研究的目的:
- 调查化对葡萄藤中DNA甲基化模式的影响.
- 比较野生葡萄和栽培葡萄的甲基化概况.
- 识别葡萄化过程中受差异甲基化影响的基因.
主要方法:
- 在18个野生和栽培葡萄藤连接上进行了减少表现的二硫酸盐测序 (RRBS).
- 不同甲基化分析以确定细胞因子甲基化变化.
- 甲基化和差异甲基化基因的功能丰富分析.
主要成果:
- 栽培葡萄树的DNA甲基化水平总体显著高于野生葡萄树.
- 在9955个差异甲基化细胞因子中,78%的细胞因子在种植的葡萄树中超甲基化.
- 核心甲基化基因与应激反应和类代谢有关;差异甲基化基因涉及过氧体向,乙烯调节和防御.
结论:
- 表观遗传变异,特别是DNA甲基化,在葡萄藤化中发挥了重要作用.
- 化改变了甲基化模式,影响了对植物适应和发育至关重要的基因.
- 在作物化过程中,Epialleles提供了一种快速表型适应的机制.
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