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在Nicotiana tabacum的叶子表面上产生工程化物

Hong Ma¹, Tyler Steede¹, Ralph E Dewey¹

¹Department of Crop and Soil Sciences, North Carolina State University, Raleigh, North Carolina 27695, United States.

Journal of agricultural and food chemistry

|June 6, 2024

概括

研究人员对烟草植物进行了改造,以产生斯克拉,这是一种用于香料的有价值化合物,具有潜在的药用益处. 这种可持续的方法克服了自然生产的局限性,为sclareol合成提供了一个新的来源.

科学领域:

植物生物技术植物生物技术
代谢工程是代谢工程.
自然产品的合成自然产品的合成

背景情况:

斯克拉勒醇是一种二烯醇,对于合成Ambrox至关重要,这是一个可持续的珀色替代品.
斯克莱罗尔具有潜在的抗菌,抗真菌和抗癌特性.
像Salvia sclarea这样的植物中斯克拉的自然丰度有限,限制了其商业可用性.

研究的目的:

为了建立一个可持续的生产方法,为sclareol.
为了研究烟草植物中异质醇生物合成的可行性.

主要方法:

克隆并对Salvia sclarea的labda-13-en-8-ol二酸盐合成酶 (LPPS) 和醇合成酶 (SS) 基因进行了特征化.
在Nicotiana tabacum (烟草) 中过度表达LPPS和SS基因,以创建转基因基因.
工程烟草植物中的量化斯克拉积累.

主要成果:

成功生成了能够进行硬质醇生物合成的转基因烟草生产线.
生产量最高的转基因菌株积累了4.1μg/cm2的sclareol.
实现了与尼科蒂亚纳丁素 (Nicotiana glutinosa) 等自然生产者相比较的斯克拉醇生产水平.

结论:

烟草中sclareol生物合成基因的异质表达是可持续sclareol生产的可行策略.
工艺烟草为商业化斯克拉醇合成提供了一个有前途的替代平台.
这种方法可以帮助满足香水和制药行业对斯克拉的需求.

关键词:

这是一种二氧化的二氧化.叶子表面叶子表面代谢工程是代谢工程.斯卡莱罗尔 (sclareol) 是一种其次代谢物二次代谢物.类类物质类物质这是一种化物化物.烟草烟草的烟草烟草是一种烟草.三合体的三合体

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