相关实验视频

Updated: Jun 24, 2025

Author Spotlight: Advancements in Stem Cell-Derived Vascularized Organoids and Optimized Isolation of Wharton's Jelly for Enhanced MSC Production

Author Spotlight: Advancements in Stem Cell-Derived Vascularized Organoids and Optimized Isolation of Wharton's Jelly for Enhanced MSC Production

Published on: July 5, 2024

从培养中获得的带衍生中介质干细胞的采集方法调节细胞特性和功能

Mitsuyoshi Nakao¹, Kenichi Nagase^1,2

¹Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo, 105-8512, Japan.

Regenerative therapy

|June 6, 2024

概括

采集人类带衍生中干细胞 (UC-MSCs) 会影响它们的功能. 与悬浮方法相比,UC-MSC表显示出优异的再粘附性和更高的细胞因子分泌,突出显示了干细胞治疗最佳收获的重要性.

科学领域:

干细胞生物学干细胞生物学
再生医学是一种再生医学.
生物技术是生物技术.

背景情况:

人类带衍生中介质干细胞 (UC-MSCs) 对于干细胞治疗至关重要.
目前培养和收获UC-MSC的方法包括酶处理,细胞除和温度降低.
这些收获技术对UC-MSC属性和功能in vitro的影响尚未完全理解.

研究的目的:

研究不同细胞采集方法对UC-MSC特性和功能的影响.
为了比较UC-MSC悬浮物和使用各种技术制备的板材.

主要方法:

UC-MSC悬浮剂使用酶治疗 (包括精酶和消散酶),细胞除和温度降低来制备.
UC-MSC 叶片在对温度有反应的盘上进行培养.
评估了细胞活力 (Annexin V染色,LDH测定),重新粘附和细胞因子分泌 (ELISA).

主要成果:

阿库塔斯治疗增加了UC-MSC亡,而细胞除损坏了细胞膜 (增加LDH).
分散显示出较低的初始细胞粘附.
与悬浮培养物相比,UC-MSC表显示出快速的重新粘附和迁移,并分泌出较高水平的关键细胞因子 (HGF,TGF-β1,IL-10,IL-6).

结论:

用于收获UC-MSCs的方法显著影响其生物活性和治疗潜力.
优化细胞采集技术,特别是UC-MSC表的使用,对于成功的干细胞治疗应用至关重要.

关键词:

单元格表格单元格表格细胞因子表达的表达酶性消化过程中的酶性消化介质细胞干细胞介质细胞干细胞.温度响应的细胞培养盘.带是一个带.

更多相关视频

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Published on: January 26, 2019

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells MSCs and Endothelial Colony Forming Progenitor Cells ECFCs

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells MSCs and Endothelial Colony Forming Progenitor Cells ECFCs

Published on: October 8, 2009

相关概念视频

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...