二维电光多光子显微镜二维电光多光子显微镜
Deano M Farinella1, Samuel Stanek2, Harishankar Jayakumar1
1University of Minnesota, Department of Neuroscience, Minneapolis, Minnesota, United States.
Neurophotonics
|June 6, 2024
概括
坦酸 (KTN) 电光衍射器 (EOD) 能够在体内进行超快速的二维 (2D) 两光子 (2P) 成像. 这项技术克服了当前用于高速神经活动监测的扫描方法的局限性.
科学领域:
- 神经科学是一个神经科学.
- 光学工程是指光学工程.
- 材料科学 材料科学 材料科学
背景情况:
- 基因编码的光指示器允许实时监测神经活动与毫秒动态.
- 现有的双光子 (2P) 成像系统由于声学和机械扫描技术接近基本物理限制而面临速度限制.
研究的目的:
- 为了评估坦酸 (KTN) 电光衍射器 (EOD) 的超快速二维 (2D) 2P成像.
- 确定KTN-EODs适用于以毫秒动态的基因编码指标的体内成像.
主要方法:
- 描述了一种商业KTN-EOD的性能,用于在体内2P显微镜的不同驱动频率和激光参数.
- 将第二个KTN-EOD集成到双轴扫描模块中,用于2D扫描.
- 通过对ASAP3的神经信号进行成像来验证该系统,ASAP3是一种遗传编码的电压指示器.
主要成果:
- 在高功率 (350 mW) 和脉冲强度 (118 fs) 的情况下,KTN-EOD实现了960 nm激光的最佳偏移.
- 单轴偏移在560kHz的线程扫描速率下,每行可产生多达32个可分辨的点.
- 双轴系统在高侧分辨率的定义视场上实现了>10kHz的速,使得ASAP3表达神经元的体内成像成为可能.
结论:
- KTN-EOD适用于超快的2P细胞成像 in vivo.
- 这项技术为开发下一代高性能显微镜提供了途径,利用基于KTN的扫描进步.
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