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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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相关实验视频

Updated: Jun 24, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

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二维电光多光子显微镜二维电光多光子显微镜.

Deano M Farinella1, Samuel Stanek2, Harishankar Jayakumar1

  • 1University of Minnesota, Department of Neuroscience, Minneapolis, Minnesota, United States.

Neurophotonics
|June 6, 2024
PubMed
概括
此摘要是机器生成的。

坦酸 (KTN) 电光衍射器 (EOD) 能够在体内进行超快速的二维 (2D) 两光子 (2P) 成像. 这项技术克服了当前用于高速神经活动监测的扫描方法的局限性.

关键词:
电光偏光器是一种电子光学偏光器.在活体中成像成像多光子显微镜的多光子显微镜电压成像 电压成像

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Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
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Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

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相关实验视频

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures
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Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
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科学领域:

  • 神经科学是一个神经科学.
  • 光学工程是指光学工程.
  • 材料科学 材料科学 材料科学

背景情况:

  • 基因编码的光指示器允许实时监测神经活动与毫秒动态.
  • 现有的双光子 (2P) 成像系统由于声学和机械扫描技术接近基本物理限制而面临速度限制.

研究的目的:

  • 为了评估坦酸 (KTN) 电光衍射器 (EOD) 的超快速二维 (2D) 2P成像.
  • 确定KTN-EODs适用于以毫秒动态的基因编码指标的体内成像.

主要方法:

  • 描述了一种商业KTN-EOD的性能,用于在体内2P显微镜的不同驱动频率和激光参数.
  • 将第二个KTN-EOD集成到双轴扫描模块中,用于2D扫描.
  • 通过对ASAP3的神经信号进行成像来验证该系统,ASAP3是一种遗传编码的电压指示器.

主要成果:

  • 在高功率 (350 mW) 和脉冲强度 (118 fs) 的情况下,KTN-EOD实现了960 nm激光的最佳偏移.
  • 单轴偏移在560kHz的线程扫描速率下,每行可产生多达32个可分辨的点.
  • 双轴系统在高侧分辨率的定义视场上实现了>10kHz的速,使得ASAP3表达神经元的体内成像成为可能.

结论:

  • KTN-EOD适用于超快的2P细胞成像 in vivo.
  • 这项技术为开发下一代高性能显微镜提供了途径,利用基于KTN的扫描进步.