暴露于迪亚佐克森会增加对Salmonella Typhimurium感染的易感性
Daniel Alberto Girón-Pérez1,2, Hannya Daryna Espinoza-Gonzalez2, Jessica Alejandra Murillo Cisneros3
1Laboratorio Nacional para la Investigación en Inocuidad Alimentaria (LANIIA)-Nayarit, Universidad Autónoma de Nayarit, Tepic, Nayarit, México.
概括
暴露于杀虫剂diazoxon增加了沙门氏菌Typhimurium感染的风险. 这项研究发现,暴露于diazoxon增加了人类细胞中的细菌进入和生长,引发了粮食安全问题.
科学领域:
- 食品安全和保障的安全和安全.
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 微生物和化学污染物影响粮食安全.
- 沙门氏菌 Typhimurium (S. Typhimurium) 导致沙门氏菌病并形成细菌储存库.
- 暴露在杀虫剂中,就像在diazoxon中一样,可以增加感染易感性.
研究的目的:
- 调查迪亚佐克森和S. Typhimurium对体外感染动态的联合作用.
- 评估前期暴露于diazoxon如何影响人类细胞S. Typhimurium内部化.
- 分析其对细胞反应的影响,如动氨酸聚合和反应性氧物种 (ROS) 生产.
主要方法:
- 人类单核细胞暴露于迪亚佐克森.
- 随后,细胞在不同的时间点 (1,8,24小时) 被S. Typhimurium挑战.
- 量化了细菌内部化,活性蛋白聚合和ROS的产生.
主要成果:
- 迪亚佐克森的预暴露导致S. Typhimurium在单核细胞中的内部化显著增加.
- 在暴露于diazoxon和S. Typhimurium的细胞中观察到ROS产量的增加.
- 观察到增强的动因聚合,促进细菌进入.
结论:
- 同时暴露于diazoxon和S. Typhimurium会增加宿主细胞对感染的易感性.
- 这些发现强调了食品中化学和微生物污染的潜在风险.
- 了解这些相互作用对于减轻食源性疾病和确保粮食安全至关重要.
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