过度负荷会通过Piezo1/endoplasmic reticulum应激通路促进关节冠状细胞亡
Xiaohui Wang1,2,3, Junli Tao1,2,3, Jianping Zhou1,2,3
1College of Stomatology, Chongqing Medical University, Chongqing, China.
Journal of cellular and molecular medicine
|June 6, 2024
概括
过度负荷会通过激活Piezo1通道引起关节 (TMJ) 骨关节炎,从而导致冠状细胞亡. 抑制Piezo1可能为TMJ骨关节炎提供治疗方法.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 骨关节炎研究 骨关节炎研究
背景情况:
- 关节 (TMJ) 的过度负荷是关节骨关节炎和软骨退化的主要驱动因素.
- 将机械负荷与关节骨关节炎联系在一起的确切机制仍然不完全理解.
- 机械激活 (MA) 离子通道,特别是Piezo1,与状细胞反应有关,并且在骨关节炎中被上调.
研究的目的:
- 为了研究Piezo1在过度的关节负荷下调解状细胞亡中的作用.
- 为了阐明在这个过程中内细胞网膜应激和离子流入的参与.
- 评估Piezo1抑制在TMJ骨关节炎中的治疗潜力.
主要方法:
- 利用一个体外模型,对肌细胞的过度肌关节负荷进行分析.
- 评估了内质网膜压力和亡标志物.
- 研究了Piezo1抑制剂GsMTx4.4的作用.
- 测量了细胞内离子的流入量.
主要成果:
- 过度负荷诱导了内质网膜应激和关节冠状细胞的亡.
- 在过度负荷下,Piezo1表达增加.
- 用GsMTx4抑制Piezo1缓解了冠状细胞亡.
- 通过离子的流入,Piezo1介导的内等质网膜应激.
结论:
- 皮埃佐1是关节关节细胞过载和亡的关键调解者.
- 通过Piezo1介导的途径涉及细胞内网膜应激和流入.
- 准Piezo1为TMJ骨关节炎提供了一个潜在的治疗策略.
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