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阿门托弗拉通过向p53信号通路轴来逆转肝细胞癌细胞中的上皮细胞-介质细胞过渡

Hui-Ying Jian¹, Jing-Ting Zhang¹, Zhuo Liu²

¹Hunan University of Chinese Medicine, Hunan, China.

Journal of cellular and molecular medicine

|June 6, 2024

概括

来自Selaginella doederleinii的阿门托弗拉通过抑制关键基因和p53通路来逆转肝细胞癌 (HCC) 的上皮细胞-介质细胞过渡 (EMT). 这种天然化合物减少了癌细胞的迁移,入侵,并诱导了亡.

科学领域:

在瘤学瘤学.
药理学药理学是指药理学的学科.
生物信息学是一种生物信息学.

背景情况:

表皮-介质细胞过渡 (EMT) 和其逆转与肝细胞癌 (HCC) 的发展有关.
传统中医药中的 Selaginella doederleinii Hieron 含有安门托弗拉,它的主要活性成分具有抗瘤特性.

研究的目的:

通过生物信息学和网络药理学研究安门托弗拉在逆转HCC中EMT的机制.
确定安门托弗拉调节的EMT基因,对HCC预后至关重要,并建立一个预测模型.

主要方法:

生物信息学和网络药理学用于选Amentoflavone调节的EMT基因并预测途径.
分子对接以评估阿门托弗拉和标的相互作用.
在体外测试 (CCK-8,伤口愈合,Transwell,流细胞计) 来评估对细胞增殖,迁移,入侵和细胞亡的影响.

主要成果:

确定了三个核心基因 (NR1I2,CDK1,CHEK1),主要在p53信号通路中进行丰富.
阿门托弗拉显著抑制了HCC细胞迁移和入侵.
阿门托弗拉在HCC细胞中诱导了亡.

结论:

阿门托弗拉通过抑制核心基因表达和阻断p53信号通路来逆转HCC中的EMT.
这种机制有效地抑制了HCC细胞的迁移和入侵.
阿门托弗拉显示出作为HCC的治疗剂的潜力.

关键词:

阿门托弗拉 (Amentoflavone) 是一种含有氨基的药物.生物信息学是一种生物信息学.表皮细胞-介质细胞过渡.肝细胞癌是肝细胞癌.网络药理学网络药理学 p53 信号通路的轴

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