Circ_0012152通过miR-652-3p/SOX4轴加速急性髓性白血病的进展
Ying Chen1,2, Bi-Xia Li2,3, Ting-Ting Niu1,2
1Laboratory of Stem Cell Transplantation, The First Affiliated Hospital of Ningbo University, Ningbo, 315300, China.
Current medical science
|June 6, 2024
概括
循环RNAcirc_0012152在急性髓性白血病 (AML) 中被上调,并预测整体存活率较低. 它通过通过miR-652-3p上调SOX4促进AML细胞生长.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 急性髓性白血病 (AML) 是一种严重的血液癌症,其特征是过度的髓性弹性增殖.
- 循环RNAs (circRNAs) 越来越多地被认为是AML发展中的角色.
- 调查 circ_0012152 等特定的 circRNA 对于理解 AML 发病过程至关重要.
研究的目的:
- 为了确定circ_0012152在AML患者中的临床意义.
- 阐明circ_0012152影响AML进展的分子机制.
主要方法:
- 使用定量实时PCR测量circ_0012152表达在247名AML患者和40名健康对照中.
- 细胞计数工具-8测定和流动细胞计量评估了细胞生长,细胞亡和细胞周期.
- RNA下拉,RNA测序和生物信息学分析确定了miRNA和mRNA的目标.
主要成果:
- 在AML患者中,Circ_0012152显著升高,被确定为整体存活期的独立不良预后因素.
- Knockdown of circ_0012152抑制了AML细胞生长,诱导了细胞亡,并停止了细胞周期的进展.
- 确定了米R-652-3p作为circ_0012152的直接标,其抑制逆转了circ_0012152敲击的效果.
- SOX4被确定为miR-652-3p的下游目标,其表达与circ_0012152水平相反相关.
结论:
- Circ_0012152 在AML中作为一个不良的预后生物标志物.
- 在circ_0012152/miR-652-3p/SOX4轴促进AML细胞的增殖和进展.
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