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转录组分析揭示了Lacticaseibacillus caseiATCC 393在响应超声波压力时的不同基因表达模式

Irene Giordano¹, Edoardo Pasolli¹, Gianluigi Mauriello¹

¹Department of Agricultural Sciences, University of Naples Federico II, 80049 Naples, Italy.

Ultrasonics sonochemistry

|June 6, 2024

概括

超声应激显著改变了Lacticaseibacillus casei中的基因表达,影响了新陈代谢和DNA稳定性. 这项研究揭示了益生菌如何对潜在的食品应用的压力做出反应.

科学领域:

微生物学微生物学
分子生物学分子生物学
食品科学食品科学食品科学

背景情况:

益生菌细菌,特别是乳酸细菌 (LAB),在食品应用中至关重要.
超声波等次致命压力可以减弱益生菌,改变它们的新陈代谢和生存策略.

研究的目的:

为了研究超声波诱导衰减后Lacticaseibacillus casei ATCC 393的转录变化.
了解益生菌应激反应和表型修饰背后的分子机制.

主要方法:

用RNA测序来分析基因表达特征.
Lacticaseibacillus casei ATCC 393接受了6分钟 (T) 和8分钟 (ST) 的超声波治疗.

主要成果:

超声波暴露导致显著的基因表达差异:在6分钟时有742个基因,在8分钟时有409个基因.
关键受影响的途径包括膜运输,碳水化合物和精氨酸代谢,菌体相关基因和翻译.
上调的基因表明能量需求增加 (糖解,酸盐代谢,纯素生物合成),而下调的基因则表明抑制蛋白质合成和严格控制DNA结构.

结论:

超声波治疗诱导了强烈的防御反应和适应在Lacticaseibacillus casei.
这些发现强调了细菌对ATP的需求,以应对压力并保持DNA完整性.
这项研究提供了对超声波对益生菌菌株及其应激反应途径的影响的见解.

关键词:

衰减减弱化减弱减弱代谢变化代谢变化益生菌是一种益生菌.有关RNA测序的RNA测序

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