使用人类肝脏显微体在实验室中对质子代谢物进行表征,并首次应用于从死亡病例中收集的两个尿液
Alice Ameline1, Laurie Gheddar1, Simona Pichini2
1Institut de Médecine Légale, 11 rue Humann, 67085 Strasbourg, France.
概括
新型合成阿片类药物Protonitazene及其代谢物在致命中毒病例的人类尿液中被发现. 检测这些代谢物可以延长对质子暴露的检测窗口.
科学领域:
- 法医毒理学 法医毒理学
- 药理学 药理学是指药理学的学科.
- 有机化学 有机化学
背景情况:
- 普罗托尼塔是一种新型合成阿片类药物,属于尼塔类,越来越多地出现在新的精神活性物质市场上.
- 尼塔与致命的中毒病例有关,这凸显了对毒理学理解的需要.
- 有限的数据存在的新陈代谢和检测的protonitazene,一个重要的知识差距.
研究的目的:
- 从致命的中毒病例中检测人类尿液中的质子及其代谢物.
- 用人肝脏显微体在体外对质子的代谢进行表征.
- 为了比较体外发现与真实样本中的体外鉴定.
主要方法:
- 超高性能液态染色学 - 协同质谱学 (UPLC-MS/MS).
- 超高性能液体染色学-高分辨率质谱学 (UPLC-HRMS).
- 在人体肝脏显微体的体外化.
主要成果:
- 在实验室中表征了protonitazene的代谢物,包括N-desethyl-protonitazene,5-amino-protonitazene和4-hydroxy-nitazene.
- 这些已识别的代谢物也被检测到来自两例致命病例的尿样中.
- 计算的代谢物与原始物比率表明了显著的代谢转化.
结论:
- 这项研究成功地在人类死亡中确定了protonitazene及其关键代谢物.
- 代谢物的表征为法医毒理学提供了关键数据.
- 检测质子代谢物可以提高暴露的检测窗口,有助于法医调查.
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