在Nellore牛的反动物微生物组中探索移动遗传元素
Camila A Faleiros1, Alanne T Nunes1, Osiel S Gonçalves2
1Department of Veterinary Medicine, School of Animal Science and Food Engineering (FZEA), University of São Paulo, Pirassununga, SP, 13635-900, Brazil.
Scientific reports
|June 6, 2024
概括
这项研究将病毒和等离子体等移动遗传元素 (MGE) 与内罗尔牛的小吃细菌联系起来. 这些发现为微生物进化和动物生产的潜在生物技术应用提供了洞察力.
科学领域:
- 微生物生态学 微生物生态学
- 基因组学就是基因组学.
- 动物科学动物科学
背景情况:
- 甲基因组学使我们能够理解动物微生物组在动物生产表型中的作用.
- 移动遗传元素 (MGE) 对于遗传物质转移和微生物进化至关重要.
- 识别牛体中MGE宿主协会对于生态和进化见解至关重要.
研究的目的:
- 为了确定在Nellore牛中反动物细菌和MGEs之间的关联.
- 调查MGEs在牧场养殖牛的反动物生态系统中的作用.
- 探索从这些微生物相互作用中获得的潜在生物技术应用.
主要方法:
- 采用了猎枪元基因组测序和ProxiMeta近距离结合方法.
- 采用Hi-C技术,在细菌和MGEs之间建立物理染色体联系.
- 组装了107个元基因组组装基因组,这些基因组来自Nellore奶牛的体样本.
主要成果:
- 确定了31种宿主细菌与MGEs之间的关联,包括17种病毒和14种等离子体链接.
- 大多数细菌基因组属于Lachnospiraceae,Bacteroidaceae,Ruminococcaceae,Sacharofermentanaceae和Treponemataceae. 这些细菌的基因组都被发现.
- 在动物微生物组中检测到12个抗生素耐药性基因.
结论:
- 这是第一个将MGEs与巴西牛中的微生物宿主联系起来的研究.
- 提供了新的洞察力,MGEs在牧场养殖的Nellore牛的羊皮.
- 这些发现可以促进生物技术的发展,以改善食品消化和反动物生产.
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