在接受心肌输液单光子发射计算机断层扫描的患者中,DNA损伤和修复
Andrea De Lorenzo1, Maria Clara Dos Santos Fernandes2, Francisco Romeiro3
1Coordenação de Ensino e Pesquisa, Instituto Nacional de Cardiologia, Rua das Laranjeiras 374, Rio de Janeiro, RJ, Brazil. andlorenzo@hotmail.com.
Scientific reports
|June 6, 2024
概括
使用 technetium-99m sestamibi 的心肌输液成像 (MPI) 并没有显著增加患者的DNA损伤. DNA修复基因表达略有增加,这表明这种诊断程序对辐射的影响最小.
科学领域:
- 放射学和核医学 放射学和核医学
- 分子生物学分子生物学
- 辐射生物学 辐射生物学
背景情况:
- 患者暴露于医学成像中的电离辐射需要进行风险评估.
- 心肌 perfusion 单光子发射计算机断层扫描 (MPS) 使用放射标志物,引发了关于潜在的DNA损伤的问题.
- 评估DNA损伤和修复标记对于理解诊断程序中的辐射效应至关重要.
研究的目的:
- 为了评估DNA损伤和修复基因表达,在心肌 perfusion 单光子发射计算机断层扫描 (MPS) 之后.
- 为了确定MPS的Tc-99m sestamibi注射是否会诱导显著的DNA链断裂.
- 为了研究MPS后DNA损伤反应途径的激活.
主要方法:
- 在Tc-99m sestamibi MPS.之前和之后,从32名患者身上采集了周围血液样本.
- 彗星测试被用来量化DNA链断裂 (DNA百分比在尾巴,尾巴长度,尾巴时刻).
- 定量PCR (qPCR) 测量了DNA损伤/修复基因 (ATM,ATR,BRCA1,CDKN1A,XPC) 的表达.
主要成果:
- 与MPS前相比,在MPS后没有观察到DNA链断裂标记的统计学上显著差异.
- 定量PCR检测显示BRCA1和XPC基因的表达增加.
- ATM,ATR和CDKN1A基因的表达水平没有显著变化.
结论:
- 一次Tc-99m sestamibi MPS程序没有诱导患者检测到的DNA链断裂.
- DNA修复基因 (BRCA1,XPC) 的有限上调表明,MPS的辐射剂量不足以触发显著的DNA损伤反应.
- 这些发现表明,从MPS诊断程序中显著DNA损伤的风险很低.
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