相关实验视频

Updated: Jun 24, 2025

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

DNA甲基化和中风预后:一个全表观基因组关联研究

Joan Jiménez-Balado¹, Isabel Fernández-Pérez^2,3, Cristina Gallego-Fábrega⁴

¹Neurovascular Research Group, Department of Neurology, Hospital del Mar Research Institute, C/ del Dr. Aiguader, 88, 08003, Barcelona, Spain. jjimenez3@imim.es.

Clinical epigenetics

|June 6, 2024

概括

DNA甲基化 (DNAm) 变异与中风预后有关. 这项研究确定了与中风不良结果相关的特定DNA甲基化标记物,可能揭示了新的恢复机制.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
神经科学是一个神经科学.
基因组学就是基因组学.

背景情况:

卒中是成年人残疾的主要原因,其结果受到临床因素和个体变化的影响.
遗传学和表观遗传学,包括DNA甲基化 (DNAm),可以解释中风预后的变化.
了解脑卒中后的表观遗传景观对于改善患者的治疗结果至关重要.

研究的目的:

研究DNA甲基化模式与中风预后之间的关联.
为了确定特定的DNA甲基化标记,预测缺血性中风后的功能结果.
探索潜在的表观遗传机制背后的中风恢复.

主要方法:

这是一项两阶段的研究,涉及发现和复制高加索血性中风患者的队列.
在中风后24小时内采集的全血样本上使用450K和EPIC BeadChips进行DNA甲基化分析.
全表观基因组关联研究 (EWAS) 以确定与功能表现差相关的差异甲基化位置 (DMPs) 和区域 (DMRs) (修改的兰金尺度>2在3个月).

主要成果:

一个复制的DMP (cg24391982) 被识别出来,被注释为血栓松丁-2 (THBS2) 基因.
四个差异甲基化区域 (DMRs) 与不良的中风结局有显著的关联.
这些DMR被注释为指蛋白57同类基因 (ZFP57),阿拉基多酸12-氧酶12S型 (ALOX12),ABI家族成员3 (ABI3) 和艾伦托依克酶 (ALLC) 基因.

结论:

在特定的DNA甲基化模式和中风结果之间存在显著的关联.
在ZFP57,ALOX12,ABI3和ALLC基因中发现的THBS2中的DMP和DMRs可能作为中风预后的潜在生物标志物.
这些发现表明了影响中风恢复的新型表观遗传机制,并突出了未来研究的途径.

关键词:

通过DNA甲基化.表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.脑卒中结局的结果血小板蛋白-2 的存在.

更多相关视频

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1

Published on: September 26, 2015

相关概念视频

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...