一种统计方法来识别导致断奶后腹猪的流行毒性因素
Chuan-Shun Lin1, Chiao-Hsia Huang2, Vincentius Surya Kurnia Adi3
1Animal Technology Research Center, Agricultural Technology Research Institute, Taiwan, R.O.C.
Veterinarni medicina
|June 7, 2024
概括
小猪的断奶后腹 (PWD) 主要是由具有F18 fimbriae和分泌热敏性肠毒素 (LT) 的肠毒性大肠杆菌菌菌株 (ETEC) 引起的. 其他毒性因子,如EAST1和Shiga毒素2e,与PWD没有显著的关联.
科学领域:
- 兽医微生物学 兽医微生物学
- 细菌病原体的产生
- 猪健康 猪健康
背景情况:
- 断奶后腹 (PWD) 在猪生产中构成了重大挑战.
- 肠毒性大肠杆菌 (ETEC) 是PWD的主要致病原体.
- 识别关键的毒性因素对于有效的疾病控制至关重要.
研究的目的:
- 在统计上确定与小猪PWD相关的ETEC菌株的流行毒性因子.
- 确定特定的ETEC毒性因子和PWD的发生之间的相关性.
主要方法:
- 从健康和腹猪中分离和描述ETEC菌株.
- 聚合酶连锁反应 (PCR) 检测六种毒性因子的基因:LT,STa,STb,EAST1,Stx2e,和F18 fimbriae.
- 与酶相关的免疫吸收试验 (ELISA) 来评估LT和ST分泌.
- 统计回归分析与PWD相关的毒性因子.
主要成果:
- 在126个ETEC菌株中没有检测到什加毒素2e (Stx2e).
- 在健康小猪的ETEC菌株中,23%分泌ST,50%携带asTA;没有一个有fedA或分泌LT.
- 在腹小猪的ETEC菌株中,LT分泌是与F18膜 (fedA) 存在相关的唯一显著因素 (P < 0.0001).
结论:
- 在PWD中普遍存在的毒性因子是ETEC菌株,它具有F18膜,分泌热可变性肠毒素 (LT).
- 在这项研究中,肠道聚合性大肠杆菌热稳定性肠毒素1 (EAST1) 和Shiga毒素2e (Stx2e) 与PWD没有显著的关联.
- 这些发现凸显了LT和F18膜在猪中ETEC介导的断奶后腹中的重要性.
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