Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Orthogonal nanopores cross-validation for multiplex single-molecule profiling.

Chemical science·2026
Same author

A Dynamic Contour Evolution Algorithm for Cell Segmentation and Synaptic Tracking under Occlusion.

Chemical & biomedical imaging·2026
Same author

Photoresponsive DNA steganography for secure information transmission by nanopore.

National science review·2026
Same author

Nanopore Fingerprinting of Neurodegenerative Proteins and Phosphoproteins within a Minute.

JACS Au·2026
Same author

Wireless nanopore electrodes (WNEs): from a non-contact conductive tip to applications in electroanalysis and electrocatalysis.

Chemical science·2026
Same author

Nanopipette Electrochemistry.

Chemical reviews·2025

相关实验视频

Updated: Jun 24, 2025

Counting Proteins in Single Cells with Addressable Droplet Microarrays
12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

8.5K

使用滴滴纳米孔的高通量单一生物标志物识别.

Lin-Lin Zhang1, Cheng-Bing Zhong1, Ting-Jing Huang1

  • 1Molecular Sensing and Imaging Center, School of Chemistry and Chemical Engineering, Nanjing University Nanjing 210023 P. R. China yilunying@nju.edu.cn.

Chemical science
|June 7, 2024
PubMed
概括

一种新的滴滴纳米孔技术使得高通量,单个生物标志物检测在图形图层面. 这种方法显著减少了样本量,促进了代谢过程的临床诊断.

更多相关视频

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
11:00

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

11.9K
The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
10:01

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

7.6K

相关实验视频

Last Updated: Jun 24, 2025

Counting Proteins in Single Cells with Addressable Droplet Microarrays
12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

8.5K
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
11:00

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

11.9K
The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
10:01

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

7.6K

科学领域:

  • 生物技术是生物技术.
  • 纳米技术纳米技术
  • 分析化学 分析化学

背景情况:

  • 生物标志物分析对于临床诊断至关重要,需要敏感的单分子检测.
  • 传统的纳米孔探测面临着由于低度和有限的并行检测而面临的低容量生物标志物的挑战.
  • 高灵敏度对于准确检测各种代谢过程中的生物标志物至关重要.

研究的目的:

  • 开发一种滴滴纳米孔技术,用于低容量,高通量单一生物标志物检测.
  • 克服传统设置在低度检测生物标志物的局限性.
  • 提高生物标志物分析的灵敏度和并行检测能力.

主要方法:

  • 在亚微升尺度上运行的滴滴纳米孔传感平台的开发.
  • 实施多通道记录,用于同时检测生物标志物.
  • 该平台的应用用于检测诸如 ангиотензин II 等生物标志物.

主要成果:

  • 与传统方法相比,样本体积减少了2000倍.
  • 证明了高吞吐量,单个生物标志物检测能力.
  • 显著降低了生物标志物的检测极限到图形图的水平 (例如,血管新生II的42 pg).

结论:

  • 滴滴纳米孔技术可以在图形图层面直接检测生物标志物.
  • 这一进步为单个分子水平的生物标志物的临床检测提供了一个有前途的解决方案.
  • 该平台最大限度地减少了对样本稀释的需求,提高了诊断效率.