通过细菌学和分子方法从北塞浦路斯的牲畜中分离和描述Brucella melitensis
Mehmet Demirpence1, Ahmet Murat Saytekin2, Baris Sareyyupoglu3
1Bacteriological Diagnosis Laboratory, Directorate of Veterinary Department, Nicosia, North Cyprus.
Veterinarni medicina
|June 7, 2024
概括
在牛,绵羊和山羊等北塞浦路斯的牲畜中通过牛奶和胎儿样本分析识别了Brucella melitensis. 这一发现凸显了菌病对公共健康的风险以及需要控制策略的必要性.
科学领域:
- 兽医微生物学 兽医微生物学
- 动物传播疾病 动物传播疾病
- 分子流行病学分子流行病学
背景情况:
- 牛病是一种严重的动物传染病,在全球范围内影响着牲畜.
- 布鲁塞拉梅利坦尼斯 (Brucella melitensis) 是小型反动物中布鲁塞洛症的主要原因,并且可以感染牛.
- 北塞浦路斯的布鲁塞洛斯病史很长,因此需要持续监测.
研究的目的:
- 在北塞浦路斯的牛,绵羊和山羊中分离,生物型和分子特征Brucella melitensis.
- 为了确定牛奶牲畜和堕胎胎儿样本中B. melitensis的流行率.
- 评估B. melitensis菌株的遗传多样性,并确定潜在的公共卫生风险.
主要方法:
- 分析了319个原始牛奶样本和32个堕胎胎儿组织样本,这些样本来自阳性乳牛.
- 使用常规细菌学方法分离和识别布鲁塞拉菌.
- 使用实时PCR和布鲁斯梯子多重PCR测定来确认分离物.
- 确认的B. melitensis分离物的生物类型.
主要成果:
- 从28.2%的牛奶样本和59.4%的胎儿样本中分离出了Brucella melitensis.
- 所有109个分离物都通过实时PCR确认为B. melitensis.
- 鉴定的分离物属于B. melitensis生物1 (74.1%) 和生物3 (25.9%) 的分离物.
- 尽管从阳性牛群中取样,但没有检测到brucella abortus.
结论:
- 证实了北塞浦路斯的牲畜,包括牛中的布鲁塞拉梅利坦尼斯的存在和传播.
- 突出了该地区与血病相关的潜在公共卫生风险.
- 强调必须实施严格的控制和根除战略,以保护动物和人类的健康.
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