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Updated: Jun 24, 2025

Single Particle Cryo-Electron Microscopy: From Sample to Structure
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Single Particle Cryo-Electron Microscopy: From Sample to Structure

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程序VUE:使用统一的球形网格分析冷EM投影的分布

Ludmila Urzhumtseva1, Charles Barchet2, Bruno P Klaholz2

  • 1Architecture et Réactivité de l'ARN, UPR 9002 CNRS, IBMC, Université de Strasbourg, 15 rue R. Descartes, 67084 Strasbourg, France.

Journal of applied crystallography
|June 7, 2024
PubMed
概括

本研究介绍了VUE,这是一个用于分析冷电子显微镜中的视图分布的程序. 它有助于识别和纠正不均的视图采样,以提高3D重建质量.

关键词:
3D重建重建的3D重建兰伯特投影是兰伯特的投影.低温电磁波冷却器 (Cryo-EM) 是一个非常好的方法.喜欢的方向,喜欢的方向.统一的网格 统一的网格查看频率 查看频率

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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