养殖系统 (子与地板) 对子皮的微生物组成和转录组的影响
Zhiyu He1,2, Xuejian Li1,2, Xi Zhang1,2
1Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Frontiers in veterinary science
|June 7, 2024
概括
养 (CR) 和地板养殖 (FR) 系统显著改变的乳腺微生物群和基因表达. 在FR中,有害细菌增加和细胞过程发生变化,这可能会影响以利亚细胞活动.
科学领域:
- 动物科学动物科学
- 微生物学 微生物学
- 基因组学就是基因组学.
背景情况:
- 从水中过渡到干旱的养殖环境.
- 不同的养殖系统,如养 (CR) 和地板养殖 (FR),可能会影响肠健康.
- 结节的微生物组成和宿主基因表达对营养吸收和整体健康至关重要.
研究的目的:
- 调查CR和FR系统对的乳腺微生物群落结构的影响.
- 分析CR和FR对的阴茎转录组的影响.
- 在不同的养殖系统下探索叶微生物群和宿主基因表达之间的潜在相关性.
主要方法:
- 使用16S rRNA基因测序 (16S rRNA-seq) 进行了叶微生物群的分析.
- 转录组测序 (RNA-seq) 用于分析阴茎粘膜中的基因表达.
- 应用了线性差异分析效应大小 (LEfSe),基因和基因组京都百科全书 (KEGG) 途径分析和斯皮尔曼相关性.
主要成果:
- CR和FR系统产生了不同的乳腺微生物组成,FR显示了更多的差异性微生物群.
- 养殖系统显著影响了微生物路径,包括氨基酸生物合成,并改变了宿主基因表达,在CR中增加了更多的基因.
- CR在氨基酸代谢途径中表现出丰富,而FR在细胞过程中表现出丰富,如亡和亡. 在FR中的特定细菌种类与参与细胞信号传递的宿主基因相关.
结论:
- 养殖系统会导致子叶微生物群和宿主基因表达的显著变化.
- 在CR和FR之间,叶叶氨基酸代谢可能会有所不同.
- 在FR系统中某些细菌的增加可能会对叶细胞功能产生负面影响.
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