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相关概念视频

Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

889
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
889
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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What is Gene Expression?01:36

What is Gene Expression?

8.5K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
8.5K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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Master Transcription Regulators02:23

Master Transcription Regulators

6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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相关实验视频

Updated: Jun 24, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
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在剂量反应氧化调节器中整合基因表达和拼接动态.

A Rasim Barutcu1, Michael B Black1, Raymond Samuel1

  • 1ScitoVation, Durham, NC, United States.

Frontiers in genetics
|June 7, 2024
PubMed
概括

专注于内子保留的替代拼接分析提供了比基因表达更敏感的方法来检测化学毒性. 这种方法通过揭示微妙的细胞反应和潜在的生物标志物来增强毒理风险评估.

科学领域:

  • 毒理学 毒理学 毒理学
  • 文字转录学 (Transcriptomics) 是一个学科.
  • 分子生物学分子生物学

背景情况:

  • 转录组学,包括基因表达和替代拼接,对于毒理风险评估至关重要,有助于确定化学品的起点 (POD) 和作用模式 (MOA).
  • 替代拼接是一种从单个基因生成多个RNA异型的过程,在细胞功能和对刺激的反应中起着至关重要的作用.

研究的目的:

  • 在毒理学评估中评估拼接失调的作用.
  • 探索替代拼接作为毒理学评估中的补充终点.
  • 调查内部保留作为化学风险评估生物标志物的潜力.

主要方法:

  • 对A549细胞进行了RNA测序 (RNA-seq),这些细胞被暴露在五种不同度的氧化应激调节剂中.
  • 进行了差异基因表达 (DGE) 分析,以确定基因表达水平的变化.
  • 替代拼接分析侧重于内部保留事件,并将基准剂量建模应用于拼接数据.

主要成果:

  • 差异性基因表达分析揭示了有限的通路丰富,主要是在高化学度下.
  • 替代拼接分析确定了所有测试化学物质的各种途径的显著内质保留事件,即使没有实质性的基因表达变化.
  • 随着化学物质暴露,内部保留事件逐渐增加,这表明拼接变化可能会先于基因表达的变化,正如在diazinon中观察到的那样.
关键词:
另一个替代拼接方法是拼接.基准剂量的基准剂量作用的作用方式.新方法方法论新方法方法论转录组学 转录组学是指转录组学.

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结论:

  • 与传统的基因表达分析相比,替代拼接分析,特别是内部保留,提供了更敏感的化学毒性测量方法.
  • 将拼接数据集成到毒基因组工具包中,可以更全面地了解转录基因组剂量反应,并提高化学风险评估.
  • 通过评估内部保留动态,分离意识毒基因组学可以识别新的生物标志物,以改善化学安全的监管决策.