对抗万胺素抗性肠球菌的监测显示,主导集群从vanA到vanB Enterococcus faecium集群的转变,丹麦,2015年至2022年
Anette M Hammerum1,2, Kasper Thystrup Karstensen1,2, Louise Roer1
1Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark.
概括
在丹麦,从2015年到2022年期间,抗胺素的肠球菌 (VRE) 和抗胺素的可变肠球菌 (VVE) 增加了. 由于治疗选择有限,对线化物耐药的VRE和VVE菌株的出现越来越令人担忧.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 在丹麦和欧洲,抗菌素的肠球菌 (VRE) 和抗菌素的变异性肠球菌 (VVE) 正在增加.
- 对于linezolid和抗万科胺素耐药性肠球菌 (LVRE) 存在有限的治疗选择.
- VVE 携带 vanA 基因复合体,但在表型上仍然对菌素敏感,这给诊断带来了挑战.
研究的目的:
- 描述2015年至2022年期间丹麦VRE和VVE的克隆转移.
- 在VRE和VVE分离物中调查基因型线化物耐药性.
主要方法:
- 4,090个丹麦临床VRE和VVE分离物的全基因组测序 (2015-2022年).
- 提取万科米抗性基因和序列类型 (STs).
- 核心基因组多部位序列类型化 (cgMLST) 针对Enterococcus faecium.
- 测试对突变或编码对线化物耐药性的基因进行测试.
主要成果:
- 99%的VRE和VVE分离物是菌.
- 从2020年开始,人们观察到从vanA转向vanB的转变,即Enterococcus faecium.
- 确定了七个主要的Enterococcus faecium集群,包括有关VVE的ST1421-CT1134 vanA. vanA.集群.
- 检测到35个对线化物耐药的VRE和8个对线化物耐药的VVE隔离物.
结论:
- 在2015年至2022年期间,丹麦的VRE和VVE发病率增加.
- 对ST1421-CT1134 vanA的VVE集群的传播是一个重大问题.
- 虽然数量较少,但因治疗选择有限而需要注意,linezolid耐药VRE和VVE的存在.
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