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Updated: Jun 24, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

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通过减少错误折叠的pegRNA相互作用来增强CRISPR主要编辑.

Weiting Zhang1,2, Karl Petri3,4, Junyan Ma1,2,5

  • 1Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, United States.

eLife
|June 7, 2024
PubMed
概括

在主要编辑指导RNA (pegRNAs) 中的内部序列互补性可以降低CRISPR主要编辑 (PE) 的效率. 简单的重新折叠程序和有针对性的突变显著提高了斑马鱼胚胎的PE效率.

关键词:
结构 RNA 结构 RNA 结构基因编辑 基因编辑遗传学 遗传学 遗传学 是一个基因组学就是基因组学.提供蛋白质的蛋白质输送.斑马鱼是一种斑马鱼.

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