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相关概念视频

Fixation and Sectioning01:03

Fixation and Sectioning

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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相关实验视频

Updated: Jun 24, 2025

Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis
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无监督的斑点增大增强了病理图像上的球体实例细分.

Fan Yang1, Qiming He1, Yanxia Wang2,3

  • 1Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen, China.

International journal of computer assisted radiology and surgery
|June 7, 2024
PubMed
概括

这项研究引入了一种无监督的染色增大方法,以改善病理图像中的球实例细分. 该技术可以提高模型在不同染料中的性能,克服传统监督方法的局限性.

关键词:
淋巴细胞细分系统的细分面具R-CNN是指一个R-CNN的面具.病理学 图像分析 图像分析斯温变压器是什么意思无监督的斑点增大方法

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Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
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Last Updated: Jun 24, 2025

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科学领域:

  • 数字病理学数字病理学
  • 医学图像分析 医学图像分析
  • 计算病理学计算病理学

背景情况:

  • 用于球实例细分的监督深度学习模型在看不见的污点上表现不佳,原因是污点特定的特征突出显示.
  • 获取多斑点注释病理学数据集是劳动密集型和耗时的,限制了模型的概括性.

研究的目的:

  • 提出一种无监督的染色增强方法,在各种染色技术中进行强大的球实例细分.
  • 增强培训数据集的染色多样性,无需手动注释.

主要方法:

  • 使用对比的未配对翻译 (CUT) 来转换周期性酸-Schiff (PAS),马森三色 (MT) 和周期性酸-银甲胺 (PASM) 染色.
  • 用Swin变压器替换了Mask R-CNN骨干,以改善实例细分中的特征提取.

主要成果:

  • 拟议的染色增强方法在PAS,PASM和MT染色的所有指标上显著优于现有的方法.
  • 废弃实验证实了拟议方法的各个组成部分的有效性.
  • 该模型在一个包含216个全幻灯片图像 (WSI) 的数据集上得到了验证.

结论:

  • 无监督的斑点增大显示出在数字病理学中改善球细分的巨大潜力.
  • 这种方法可以扩展到病理图像分析中的其他复杂的细分任务.