在出生后的舌头发育和成年人恒常状态期间,小鼠口味系统中的刺信号元件的明显表达模式
Archana Kumari1, Nicole E Franks2, Libo Li1
1Department of Biologic and Materials Sciences & Prosthodontics, School of Dentistry, University of Michigan, Ann Arbor, Michigan, United States of America.
PloS one
|June 7, 2024
概括
刺 (HH) 途径对于味觉器官的发育和维护至关重要. 这项研究在产后发育期间绘制了小鼠舌头乳头中的HH通路组件,揭示了对味蕾成熟至关重要的差异性表达模式.
科学领域:
- 发展生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 刺 (HH) 途径对于胚胎发育和成人的味觉器官再生至关重要,包括真菌状 (FP),环状 (CVP) 和叶状 (FOP) 乳头.
- 关于在产后味觉器官分化和成熟期间的HH通路组件表达的知识缺口存在,特别是GLI转录因子和HH受体.
研究的目的:
- 绘制HH通路组件的表达图,包括连接体,受体和转录效应因子,在小鼠FP,CVP和FOP的早期和晚期产后和成年阶段.
- 为了研究这些成分在成年人的软 palates 和相关的头骨节中的表达.
主要方法:
- 使用lacZ报告者小鼠模型可视化和绘制Sonic Hedgehog (SHH) 配体,HH受体 (PTCH1,GAS1,CDON,HHIP) 和GLI转录因子 (GLI1,GLI2,GLI3) 的表达模式.
- 在不同产后和成年人发育阶段检查了真菌状乳头 (FP),环状乳头 (CVP) 和叶状乳头 (FOP) 的表达.
- 还分析了成年小鼠的软 palatal 和生殖器和三腺节的表达.
主要成果:
- 在所有三种乳头类型和软的味蕾中,SHH和GAS1在味蕾中得到一致的表达.
- 产后早期阶段在FP表皮,肌层和肌肉中表现出广泛的HH信号成分表达,在后期阶段,特定的成分如GLI1和GAS1被降低或消除.
- 与前面的FP相比,在CVP和FOP表皮中观察到HH受体的明显表达模式,并且在头骨中只发现了积极的HH信号调节器.
结论:
- 在不同的产后发育期以及前后味觉器官之间,HH信号的调节有所不同.
- 该研究提供了HH通路组件的全面表达图,为未来关于它们在产后舌头发育和再生中的作用的功能研究奠定了基础.
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