相关实验视频

Updated: Jun 24, 2025

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Published on: July 13, 2018

ITFG2是一种免疫调节蛋白,准ATP 5b以维持心肌梗塞中线粒体功能

Fang-Fang Bi¹, Miao Cao¹, Qing-Ming Pan¹

¹Department of Pharmacology (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, PR China.

Biochemical pharmacology

|June 7, 2024

概括

免疫蛋白ITFG2通过维护线粒体功能来保护心脏免受伤害. 过度表达ITFG2可以减少心脏病发作的大小,改善心脏功能,为心肌梗塞提供新的临床策略.

科学领域:

心血管生物学心血管生物学
分子心脏病学分子心脏病学
线粒体医学线粒体医学

背景情况:

ITFG2是一种细胞内蛋白调节B细胞命运和mTORC1信号.
尽管心脏表达率高,但其在心脏病中的作用仍然很大程度上未知.

研究的目的:

研究ITFG2在心肌梗塞 (MI) 和心脏功能障碍中的病理生理作用.
阐明ITFG2心脏保护作用的基础分子机制.

主要方法:

腺相关病毒血清型9 (AAV9) 载体用于小鼠ITFG2过度表达.
短发针RNA (shRNA) 和小干扰RNA (siRNA) 用于ITFG2的淘汰.
心脏特异性转基因小鼠模型.
在实验室中使用低毒新生儿大鼠心肌细胞 (NMCMs) 的研究.
评估心脏病发作的大小,心脏功能 (排泄分数,分数缩短),线粒体功能 (ATP,ROS,MMP) 和蛋白质相互作用.

主要成果:

AAV9介导的ITFG2过度表达减少了心脏病发作的大小,改善了小鼠心脏病发作中的心脏功能.
ITFG2倒置加重缺血引起的心脏功能障碍.
心脏特异性ITFG2转基因小鼠在MI后表现出较小的心脏病发作和更好的心脏功能.
通过增加ATP,减少ROS和保持MMP,ITFG2在缺氧心肌细胞中保留了线粒体功能.
ITFG2与NEDD4-2和ATP 5b形成一个复合体,抑制NEDD4-2的ATP 5b的无处不在.

结论:

ITFG2对缺血性损伤具有显著的心脏保护作用.
ITFG2通过增强线粒体弹性和调节ATP 5b稳定性来保护心脏功能.
ITFG2是治疗心肌梗塞的一个有希望的治疗点.

关键词:

在ATP合成酶子单元-β (ATP 5b) 中.根据ITFG2的规定.线粒体功能线粒体功能心肌梗塞的心脏病发作这是NEDD4-24-2的结果.在Ubiquitination中使用.

更多相关视频

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Published on: May 26, 2023

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

Published on: January 19, 2017

相关概念视频

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

ATP Synthase: Mechanism

ATP Synthase: Mechanism

In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...

Translocation of Proteins into the Mitochondria

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...

The Inner Mitochondrial Membrane

The Inner Mitochondrial Membrane

The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria. In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...

Energy to Drive Translocation

Energy to Drive Translocation

Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...

Mitochondrial Protein Sorting

Mitochondrial Protein Sorting

Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death. Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...