雷奥病毒感染诱导转录组范围独特的A-to-I编辑变化在小鼠纤维细胞
Ayesha Tariq1, Helen Piontkivska2
1Department of Biological Sciences, Kent State University, Kent, OH, USA.
Virus research
|June 7, 2024
概括
雷奥病毒感染改变了整个转录组的宿主RNA编辑模式,揭示了作用于RNA (ADAR) 点的腺氨酸脱氨酶的动态变化. 这些修改会影响免疫调节和细胞通路.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 遗传学 遗传学 是一个
背景情况:
- 氨酸对氨酸 (A-to-I) RNA编辑由氨酸脱氨酶作用于RNA (ADARs) 对于蛋白质组多样性和免疫反应至关重要.
- 包括ADARp150在内的ADARs在病毒感染期间被激活,但它们对宿主转录组范围内的编辑模式的具体影响仍然不完全理解.
- 之前关于reovirus感染的研究表明微妙的编辑,但仅限于少数网站.
研究的目的:
- 为了研究转录组范围内的reovirus感染对宿主ADAR编辑模式的影响.
- 为了确定受感染诱导的RNA编辑变化影响的特定基因和途径.
主要方法:
- 来自感染野生类型和突变类型reoviruses的小鼠纤维细胞的深度测序RNA-seq数据集的分析.
- 在受感染的样本和对照样本中对RNA编辑部位变化的全转录组检查.
主要成果:
- 转录病毒感染会在整个转录组的宿主RNA编辑模式中引发独特的细微变化.
- 在受感染的样本中发现了新的编辑部位,这些部位在未受感染的细胞中不存在.
- 改变编辑部位的基因在免疫调节,细胞信号传递,新陈代谢和生长途径方面得到了显著的丰富.
- 观察到编辑目标的转变,编辑速率和感染和控制条件之间的网站特异性差异.
结论:
- 雷奥病毒感染以特定条件的方式动态调节宿主ADAR编辑.
- 这些发现强调了RNA编辑在宿主病毒相互作用和细胞反应中的重要作用.
- 这项研究提供了第一个转录组全方位的视图,显示了继转录病毒感染后宿主编辑的变化.
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