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在丸细胞上诱导的超偏磁铁氧化物纳米颗粒的毒性:体外试验研究研究

Graziela de P F Dantas¹, Fausto S Ferraz¹, John L P Coimbra¹

¹Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.

|June 7, 2024

概括

超偏磁铁氧化物纳米粒子 (SPIONs) 对丸和精子细胞具有毒性,诱导氧化应激. 需要进一步的研究,以确保这些纳米粒子的繁殖纳米安全.

科学领域:

生物医学研究的研究.
纳米技术纳米技术
生殖毒理学生殖毒理学

背景情况:

超偏磁铁氧化物纳米粒子 (SPIONs) 广泛用于生物医学应用.
有限的数据存在于SPIONs的生殖毒性,特别是丸细胞.

研究的目的:

研究酸涂层SPIONs (Cit_SPIONs) 对小鼠初级丸细胞,丸碎片和精子的体内细胞毒性作用.
评估Cit_SPIONs对精子参数和氧化应激标志物的影响.

主要方法:

合成和Cit_SPIONs的物理化学表征.
丸细胞和精子在体外暴露于Cit_SPIONs.
评估细胞活力,精子运动性,DNA完整性,线粒体活动,脂质过氧化和ROS生产.
传输电子显微镜用于细胞吸收分析.

主要成果:

酸涂层提高了SPIONs的尺寸和稳定性,并且在3%的生物相容性.
Cit_SPIONs对丸体细胞和精子细胞表现出细胞毒性.
莱迪格细胞优先内化Cit_SPIONs,导致细胞质变化.
暴露于SPIONs对精子运动,活力,DNA完整性,线粒体活性,脂质过氧化和ROS产生产生了负面影响.

结论:

丸体和精子细胞对Cit_SPIONs敏感,引起氧化应激.
间对男性生殖系统构成潜在威胁.
铁氧化物纳米颗粒的开发必须优先考虑生殖纳米安全.

关键词:

纳米毒性纳米毒性氧化应激是一种氧化应激.间们就是间.丸细胞是一种丸细胞.超结构性变化超结构性变化

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