Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Child and Adolescent Psychiatry in the Far East: Findings from Multi-National Surveys.

Current psychiatry reports·2026

Same author

Mapping and engineering the human cell-cell interactome.

Nature biotechnology·2026

Same author

Expeditionary Point-of-Care Ultrasound for Combat and Austere Environments.

Journal of special operations medicine : a peer reviewed journal for SOF medical professionals·2026

Same author

An injectable hydrogen-producing bacteria hydrogel for cardiac repair in rodent and porcine models.

Nature biomedical engineering·2026

Same author

Autonomous Uncertainty Quantification for Computational Point-of-Care Sensors.

ACS nano·2026

Same author

Active protein-capturing hydrogel for broad-spectrum pathogen defense.

Science advances·2026

Search research articles

相关实验视频

Updated: Jun 24, 2025

Setting a Successful Sorting for Extracellular Vesicle Isolation

Setting a Successful Sorting for Extracellular Vesicle Isolation

Published on: October 11, 2024

优化细胞疗法通过分类具有高细胞外囊分泌的细胞来优化细胞疗法.

Doyeon Koo¹, Xiao Cheng^2,3, Shreya Udani¹

¹Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.

Nature communications

|June 7, 2024

概括

使用纳米技术选择高细胞外囊 (EV) 分泌的介质干细胞 (MSCs),提高了心脏病发作模型的治疗疗效. 这种方法通过丰富强大的EV生产细胞来增强再生细胞疗法.

更多相关视频

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

Published on: August 16, 2024

相关实验视频

Last Updated: Jun 24, 2025

Setting a Successful Sorting for Extracellular Vesicle Isolation

Setting a Successful Sorting for Extracellular Vesicle Isolation

Published on: October 11, 2024

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

Published on: August 16, 2024

科学领域:

再生医学是一种再生医学.
生物技术是生物技术.
细胞疗法细胞疗法

背景情况:

基于细胞外囊泡 (EV) 的疗法的临床转化面临挑战,因为缺乏丰富高EV分泌细胞的方法.
目前的细胞分类技术依赖于表面标记,这些标记与EV分泌水平或治疗潜力无关.

研究的目的:

开发和应用一种纳米技术,以基于单细胞的EV分泌率来丰富单细胞.
为了研究高EV分泌介质干细胞 (MSCs) 对于再生医学的治疗潜力.

主要方法:

利用纳米技术,基于EV分泌物分离和丰富数百万个单细胞.
应用该技术来选择高EV分泌的MSC.
分析了选定的MSC的转录形状.
在心肌梗塞的小鼠模型中评估了高分泌MSCs的疗效.

主要成果:

在使用纳米技术的基础上,成功丰富了数百万个单细胞的EV分泌.
在与EV生物发生和血管再生相关的高分泌MSC中确定了不同的转录特征.
证明,选择的MSC在分类和再生后保持高EV分泌量.
在接受高分泌MSCs治疗的小鼠中,与低分泌MSCs在心肌梗塞后治疗的小鼠中表现出改善的心脏功能.

结论:

选择基于EV分泌的细胞是一种可行的策略,可以提高再生细胞疗法的疗效.
纳米技术为治疗应用提供了一种用于丰富强大的EV分泌细胞的新方法.
EV分泌是MSC治疗疗法的治疗成功的一个关键因素.