简单而强大的3D MINFLUX激发与可变相板的激发
Takahiro Deguchi1, Jonas Ries2,3,4,5
1European Molecular Biology Laboratory, Cell Biology and Biophysics, Heidelberg, Germany.
Light, science & applications
|June 7, 2024
概括
本研究介绍了一种更快,更强大,更具成本效益的模块,用于MINFLUX (Minimal reMoval of FoLd structure) 的超分辨率成像. 新设计能够快速扫描和切换激发模式,以改善单一光体定位.
科学领域:
- 光学显微镜是一种光学显微镜.
- 纳米技术纳米技术
- 生物物理学的生物物理.
背景情况:
- MINFLUX (Minimal reMoval of foLd structure) 显微镜为超分辨率成像和单一光子跟踪提供了卓越的分辨率.
- 现有的MINFLUX实现是复杂的,昂贵的,速度和稳定性有限的.
研究的目的:
- 为MINFLUX显微镜开发一种高性能,高性价比,强大的激发模块.
- 通过快速相位扫描来提高MINFLUX本地化速度和多功能性.
主要方法:
- 使用电光调制器与空间光调制器相结合,创建一个可变相位板.
- 生成的MHz时间尺度相位扫描用于单个光体的快速探测.
- 开发了三维中紧的激发点扩散函数的两截式和顶帽相位模式.
主要成果:
- 在光体中实现了微秒时间尺度扫描激发模式.
- 已证明60微秒内的切换时间和激发波长之间的交替.
- 展示了一个强大的3D和多色MINFLUX激发模块设计.
结论:
- 开发的模块显著提高了MINFLUX的速度,稳定性和成本效益.
- 这一创新为更广泛采用高性能开源MINFLUX系统铺平了道路.
- 该模块被设想为未来先进显微镜平台的关键组件.
相关概念视频
Time and frequency -Domain Interpretation of Phase-lead Control
80
Phase-lead controllers are commonly used in various control systems to enhance response speed and stability. Adjusting the brightness on a television screen offers a practical example of phase-lead control. When contrast is enhanced, a phase-lead controller is employed. Mathematically, phase-lead control is identified when the first parameter is smaller than the second.
The design of phase-lead control involves the strategic placement of poles and zeros to balance steady-state error and system...
The design of phase-lead control involves the strategic placement of poles and zeros to balance steady-state error and system...
80
Phase-lead and Phase-lag Controllers
167
Understanding the working function of different types of controllers can be illustrated with practical analogies, such as adjusting a stereo's volume equalizer. Cranking up the bass involves a phase-lead controller, which functions as a high-pass filter, while increasing the treble uses a phase-lag controller, which acts as a low-pass filter. PD controllers, similar to high-pass filters, enhance the system's response to high-frequency components. PI controllers, akin to low-pass...
167
Time and frequency -Domain Interpretation of Phase-lag Control
88
Phase-lag controllers are widely used in control systems to improve stability and reduce steady-state errors. A dimmer switch controlling the brightness of a light bulb serves as a practical example of phase-lag control, gradually adjusting the bulb's brightness. Mathematically, phase-lag control or low-pass filtering is represented when the factor 'a' is less than 1.
Phase-lag controllers do not place a pole at zero, but instead influence the steady-state error by amplifying any...
Phase-lag controllers do not place a pole at zero, but instead influence the steady-state error by amplifying any...
88


