3D MINFLUX

Takahiro Deguchi1, Jonas Ries2,3,4,5

  • 1European Molecular Biology Laboratory, Cell Biology and Biophysics, Heidelberg, Germany.

概括

本研究介绍了一种更快,更强大,更具成本效益的模块,用于MINFLUX (Minimal reMoval of FoLd structure) 的超分辨率成像. 新设计能够快速扫描和切换激发模式,以改善单一光体定位.

相关概念视频

Time and frequency -Domain Interpretation of Phase-lead Control01:24

Time and frequency -Domain Interpretation of Phase-lead Control

Phase-lead controllers are commonly used in various control systems to enhance response speed and stability. Adjusting the brightness on a television screen offers a practical example of phase-lead control. When contrast is enhanced, a phase-lead controller is employed. Mathematically, phase-lead control is identified when the first parameter is smaller than the second.
The design of phase-lead control involves the strategic placement of poles and zeros to balance steady-state error and system...
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Phase-lead and Phase-lag Controllers01:22

Phase-lead and Phase-lag Controllers

Understanding the working function of different types of controllers can be illustrated with practical analogies, such as adjusting a stereo's volume equalizer. Cranking up the bass involves a phase-lead controller, which functions as a high-pass filter, while increasing the treble uses a phase-lag controller, which acts as a low-pass filter. PD controllers, similar to high-pass filters, enhance the system's response to high-frequency components. PI controllers, akin to low-pass...
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Time and frequency -Domain Interpretation of Phase-lag Control01:21

Time and frequency -Domain Interpretation of Phase-lag Control

Phase-lag controllers are widely used in control systems to improve stability and reduce steady-state errors. A dimmer switch controlling the brightness of a light bulb serves as a practical example of phase-lag control, gradually adjusting the bulb's brightness. Mathematically, phase-lag control or low-pass filtering is represented when the factor 'a' is less than 1.
Phase-lag controllers do not place a pole at zero, but instead influence the steady-state error by amplifying any...
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