DLG1在SDCCAG3和IFT20的上游运作,以控制多素-2的状向
Csenge K Rezi1, Mariam G Aslanyan2, Gaurav D Diwan3,4
1Department of Biology, University of Copenhagen, Copenhagen, Denmark.
EMBO reports
|June 7, 2024
概括
在细胞中破坏DLG1会损害状蛋白质的运输,导致囊. 与脏异常相关的特定DLG1变异无法恢复正常的状功能.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 腎臟病學 (nephrology) 是一種醫學專業.
背景情况:
- 极化囊泡流通对于状细胞功能至关重要,但其调节仍然不清楚.
- 包括DLG1在内的Scribble极性复合体与细胞极性有关.
- 乳在脏的发育和功能中起着至关重要的作用.
研究的目的:
- 调查DLG1在调节皮上皮细胞中状蛋白贩运中的作用.
- 为了确定受DLG1损失影响的蛋白质.
- 为了探索与CAKUT相关的DLG1变异的功能影响.
主要方法:
- 在小鼠脏中条件淘汰Dlg1.
- 基于细胞的近距离标记蛋白质组学.
- 光显微镜 光显微镜.
- 生物化学方法和阿尔法折叠模型.
主要成果:
- 在小鼠脏中,DLG1的淘汰会导致状腺的延长和细胞生成.
- DLG1的损失改变了状蛋白质组,降低了SDCCAG3,IFT20和多素-2 (PC2) 的水平.
- 一种与CAKUT相关的DLG1变体 (p.T489R) 无法挽救观察到的毛缺陷.
结论:
- DLG1对于维持皮上皮细胞中正常的状蛋白组成至关重要.
- SDCCAG3和IFT20形成与DLG1相关的复合体,影响着状细胞的贩运.
- 由于DLG1 p.T489R变异的状功能障碍可能导致脏和尿路的先天性异常 (CAKUT).
关键词:
在 DLG1 里面,你会看到 DLG1 .国际金融基金20 IFT20聚素-2 是一种多素.主要的小毛.SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3 SDCCAG3更多相关视频
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