一种瘤增强剂通过调节ETV4表达来促进黑色素瘤的进展
Junyou Zhang1,2,3, Qilin Wang1,2,3, Sihan Qi1,2,3
1School of Engineering Medicine, Beihang University, Beijing, 100191, China.
Journal of translational medicine
|June 7, 2024
概括
一种新型增强剂enh17通过调节ETV4表达来促进黑色素瘤的进展. 准这种增强剂为黑色素瘤治疗提供了一个潜在的策略.
科学领域:
- 基因组学就是基因组学.
- 癌症生物学 癌症生物学
- 分子瘤学分子瘤学
背景情况:
- 增强剂是关键的基因调控元素,涉及到发育和疾病.
- 异常增强剂可以影响癌症风险和瘤基因激活,但它们在癌症中的机制往往不清楚.
- 这项研究研究了一种特定增强剂在黑色素瘤中的作用.
研究的目的:
- 为了识别和表征一种参与黑色素瘤进展的新增剂.
- 阐明这种增强剂调节目标基因的分子机制.
- 探索向这种增强剂在黑色素瘤中的治疗潜力.
主要方法:
- 多omics数据分析以确定增强剂 (enh17).
- 在CRISPR/Cas9基因编辑中删除黑色素瘤细胞中的ENH17.
- RNA-seq,ChIP-seq,Hi-C和露西法酶记者测定用于识别目标基因和转录因子.
- 在体外和体外功能实验以验证发现.
主要成果:
- 在黑色素瘤中发现了一种异常激活的增强剂 enh17.
- 删除enh17显著抑制了黑色素瘤细胞的增殖,迁移和瘤生长.
- ETV4被确定为由 enh17调节的直接点基因,其中STAT3作为结合转录因子.
结论:
- 增强剂ehn17在黑色素瘤进展中起着至关重要的瘤作用.
- 该研究充分阐明了ehn17的转录调节机制,涉及STAT3和ETV4.
- 针对 enh17 提出了一个有前途的治疗策略,用于预防和治疗黑色素瘤.
相关概念视频
Induced Pluripotent Stem Cells
4.0K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.0K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
Cancer-Critical Genes I: Proto-oncogenes
8.8K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.8K
Mitogens and the Cell Cycle
6.5K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.5K
The Tumor Microenvironment
6.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.6K
Rous Sarcoma Virus (RSV) and Cancer
5.1K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
5.1K


