Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
RNA Interference01:23

RNA Interference

26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
Nucleic Acid Structure01:25

Nucleic Acid Structure

6.1K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
6.1K
pre-mRNA Processing02:01

pre-mRNA Processing

52.8K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
52.8K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

6.8K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.8K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

16.7K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

SPSignal: a web tool for structure-assisted prediction of nuclear localization and nuclear export signals in proteins.

Nucleic acids research·2026
Same author

Extracellular Small RNAs in Human Milk: Molecular Profiles, Stability and Fragment-Specific Responses in Cell-Based Assays.

Non-coding RNA·2026
Same author

Transcriptome-wide analysis of Arabidopsis DICER-LIKE1 RNA substrates.

Nucleic acids research·2026
Same author

Nuclear Argonaute HRDE-1 Maintains Chromatin-Independent Transcriptional Silencing through a Transgenerational Small RNA Feedback Loop.

bioRxiv : the preprint server for biology·2025
Same author

The Histidine Kinase VraS Interacts with Vancomycin and Penicillins Through its Membrane-Anchored N-terminal Domain.

Angewandte Chemie (International ed. in English)·2025
Same author

H3K27me3 expands the epigenetic control of piRNA transcription.

Nature structural & molecular biology·2025
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
查看所有相关文章

相关实验视频

Updated: Jun 24, 2025

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.5K

在植物MIRNA加工中,miRNA/miRNA*功能原理.

Santiago Rosatti1, Arantxa M L Rojas1, Belén Moro1,2

  • 1Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Universidad Nacional de Rosario, Rosario, Santa Fe, 2000, Argentina.

Nucleic acids research
|June 8, 2024
PubMed
概括
此摘要是机器生成的。

植物微RNA (miRNA) 的处理取决于miRNA/miRNA*双重结构. 优化这种双重增强miRNA水平,揭示结构,功能和进化之间的联系.

更多相关视频

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

8.3K
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

10.6K

相关实验视频

Last Updated: Jun 24, 2025

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.5K
A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

8.3K
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

10.6K

科学领域:

  • 植物分子生物学 植物分子生物学
  • 基因调节 基因调节
  • 这就是RNA生物生成.

背景情况:

  • 微RNAs (miRNAs) 是重要的基因表达调节者.
  • 植物miRNA生物发生包括通过DICER-LIKE1 (DCL1) 来精确切除折叠前体.
  • 植物miRNA前体表现出各种尺寸和形状,处理以基点到循环或循环到基点的方向发生.

研究的目的:

  • 为了研究miRNA/miRNA*双重区域在植物miRNA前体加工中的作用.
  • 了解双重结构如何影响miRNA水平和处理效率.
  • 将miRNA/miRNA*结构与前体处理机制和进化联系起来.

主要方法:

  • 在体内对内源性miRNA前体的分析.
  • 研究miRNA/miRNA*双重修改对处理的影响.
  • 对比两步和连续MIRNA的处理要求.

主要成果:

  • 由于miRNA/miRNA*复合体的不匹配,内源性miRNAs的低最佳处理在体内发生.
  • 在两步MIRNA中增强miRNA/miRNA*双重体的相互作用能量,可显著增加miRNA水平.
  • 序列MIRNA表现出独特的处理要求,这取决于特定的miRNA/miRNA*双重特征.

结论:

  • 这种miRNA/miRNA*双重结构极大地影响了植物miRNA前体的处理.
  • 双重区域的修改可以调节miRNA水平.
  • 这项研究将miRNA/miRNA*结构,加工机制,生物功能和植物进化模式联系起来.