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肥胖子宫中DNA甲基化和Dnmt表达的改变可能导致植入失败

Nazlican Bozdemir¹, Tuba Kablan¹, Mehmet Ozgen Altintas^2,3

¹Department of Histology and Embryology, Ankara Medipol University School of Medicine, Ankara, 06050, Turkey.

Journal of molecular histology

|June 8, 2024

概括

由高脂肪饮食引起的肥胖会显著改变小鼠子宫中的DNA甲基化和DNA甲基转移酶 (Dnmts). 这种表观遗传失调,特别是在子宫内膜,可能导致异常的基因表达,并影响生殖健康.

科学领域:

生殖生物学生殖生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
代谢障碍代谢障碍代谢障碍

背景情况:

肥胖是生殖问题的重要危险因素.
表观遗传修饰,特别是DNA甲基化,在基因调节中起着至关重要的作用.
以前的研究将肥胖与小鼠生殖腺的DNA甲基化改变联系在一起,但子宫效应仍未研究.

研究的目的:

研究高脂肪饮食 (HFD) 对大鼠子宫中的DNA甲基转移酶 (Dnmts) 和全球DNA甲基化的影响.
确定HFD诱导的肥胖如何影响子宫组织中Dnmt1,Dnmt3a,Dnmt3b和Dnmt3l的表达.

主要方法:

在使用高脂肪饮食 (HFD) 的小鼠中诱导肥胖.
在子宫组织中分析Dnmt1,Dnmt3a,Dnmt3b和Dnmt3l的表达水平.
全球DNA甲基化水平在子宫中的量化.

主要成果:

HFD显著改变了子宫中的Dnmt水平和全球DNA甲基化.
Dnmt1,Dnmt3a和Dnmt3b的总表达上调,而Dnmt3l和全球DNA甲基化下降.
Dnmt3b和Dnmt3l的表达在子宫内膜 (腺体和上皮质) 中增加,但随着全球DNA甲基化,在肌膜和肌膜中减少.

结论:

由HFD诱导的肥胖会大大改变小鼠子宫中的全球DNA甲基化和Dnmt表达.
减少DNA甲基化和改变Dnmt表达在子宫,特别是子宫内膜,可能导致异常的基因表达.
这些表观遗传变化凸显了一个潜在的机制,将肥胖与生殖功能障碍联系在一起.

关键词:

通过DNA甲基化.在 Dnmt 中.有高脂肪的饮食.肥胖问题肥胖问题在子宫,子宫.

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