通过GATA转录因子AaaAreA在Alternaria alternata中调节利用和真菌毒素生物合成
Liuqing Wang1, Cheng Wang2, Liang Xu3
1Institute of Quality Standard and Testing Technology of BAAFS (Beijing Academy of Agriculture and Forestry Sciences), No. 9 Middle Road of Shuguanghuayuan, Haidian District, Beijing, 100097, China.
GATA转录因子AreA调节了Alternaria alternata中的真菌毒素的产生. 它的缺失会减少生长和真菌毒素生物合成,特别是在特定的源下,如NaNO3.
科学领域:
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
- 食品科学 食品科学 食品科学
背景情况:
- 替代菌 (Alternaria alternata) 是一个重要的收获后病原体,产生有害的真菌毒素,如替代醇 (AOH) 和替代醇单甲基乙烯 (AME).
- 的可用性极大地影响了真菌生长和二次代谢物产生,包括菌毒素.
- 已知GATA转录因子AreA调节真菌中的利用,但其在A. alternata菌毒素生物合成中的特定作用在很大程度上未被描述.
研究的目的:
- 阐明AaaAreA转录因子在Alternaria alternata中的AOH和AME生物合成上的调节机制.
- 研究不同源对真菌生长,化和真菌毒素积累的影响.
- 了解AaaAreA在控制菌毒素生产中的转录调节途径.
主要方法:
- 在不同源下 (NH4Cl与NaNO3) 进行真菌生长,结合和菌毒素生产的比较分析.
- 构建和分析AaaAreA基因淘汰突变体,以评估其在利用和真菌毒素生物合成中的作用.
- 对AaAreA.的亚细胞局部化研究.
- 转录组分析以确定由AaaAreA.调节的下游目标基因.
- 电泳运动转移试验 (EMSA) 验证了AaaAreA与aohR基因的促进体的直接结合.
主要成果:
- 与主要来源NH4Cl相比,二次源NaNO3显著增强了菌根的生长,化和菌毒素的积累.
- 在大多数源上,除了NH4Cl,AaaAreA淘汰突变体表现出减少的生长,并显著损害了真菌毒素生物合成.
- 发现AaaAreA可以积极调节AOH和AME生物合成,在NaNO3.3下核局部化得到增强.
- 转录组数据显示,AaaAreA的删除降低了关键的真菌毒素生物合成基因的调节,包括aohR,pksI和omtI.
- AaAreA直接与aohR的促进子区域结合,aohR是一种特定途径的转录因子,激活其表达,并随后影响下游合成酶基因.
结论:
- 在Alternaria alternata中,AaaAreA在替代醇 (AOH) 和替代醇单甲基乙烯 (AME) 的生物合成中起着至关重要的积极调节作用.
- 源显著影响AaAreA的调节功能和亚细胞局部化,影响菌毒素的产生.
- AaAreA直接激活转录因子aohR,这反过来又控制了真菌毒素生物合成基因的表达,建立了一个关键的调节途径.
- 了解这种AaaAreA介导的监管途径对于开发有效的策略来控制农产品中的真菌毒素污染至关重要.
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