基于眼球形的凝微球构建圆顶形的3D角膜上皮组织模型
Yilan Wang1, Feng Yang1, Menghan Yang1
1School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031, China.
ACS applied materials & interfaces
|June 8, 2024
概括
研究人员使用眼球形状的凝微球开发了一种新的3D角膜模型. 这种先进的模型模仿了人类的角膜结构和功能,为眼科疾病研究提供了新的可能性.
科学领域:
- 生物材料科学 生物材料科学
- 眼科医生 眼科 眼科
- 组织工程是组织工程.
背景情况:
- 在眼科研究中,体外三维 (3D) 角膜模型至关重要.
- 现有的模型缺乏人角膜的生理圆顶形状和可调整的曲率.
- 对当前模型来说,复制体内微环境仍然是一个挑战.
研究的目的:
- 开发一种新的3D体外角膜模型,精确模仿人类角膜结构.
- 创建一个生理学上相关的模型,可调节曲率和圆顶形状.
- 建立一个研究眼科疾病和对刺激的反应的平台.
主要方法:
- 使用双相水系统制造原/奇托桑-酸眼球形凝微球.
- 通过在微球上播种人类角膜上皮细胞,构建3D角膜上皮组织模型.
- 微球曲率的调整通过改变原/奇托桑与酸盐滴的直径比.
- 评估模型对UVB暴露的反应,包括活性氧物种 (ROS) 和促炎因子分泌.
主要成果:
- 成功制造出具有可调节曲率的眼球形凝微球.
- 在体外形成的3D角膜上皮组织,呈现圆顶状的多层和紧密的结合点.
- 证明模型对UVB暴露的反应性,由增加的ROS和促炎因子分泌量表明.
结论:
- 开发的3D角膜上皮组织模型具有圆顶形状,是眼科研究的重大进展.
- 这种新型模型有效地复制了人类角膜环境的关键方面.
- 该模型显示了研究疾病机制和眼科治疗干预的潜力.
相关概念视频
01:17Accessory Structures of the Eye
Optical perception, or vision, is an extraordinary sense dependent on converting light signals received via the ocular organs. These organs, known as eyes, are securely positioned within the bony cavities of the skull, called orbits. The orbits serve a dual purpose: a protective shield for the ocular globes and a stable attachment point for the soft ocular tissues. The eye's external protective mechanisms include the eyelids, which are edged with lashes that act as a barrier against foreign...
01:20Anatomy of the Eyeball
The eye is a spherical, hollow structure composed of three tissue layers. The outer layer — the fibrous tunic, comprises the sclera — a white structure — and the cornea, which is transparent. The sclera encompasses some of the ocular surface, most of which is not visible. However, the 'white of the eye' is distinctively visible in humans compared to other species. The cornea, a clear covering at the front of the eye, enables light penetration. The eye's middle layer, the vascular tunic,...