OsWRKY12通过向大米中不同的下游转录因子基因来负面调节干旱应激耐受性和二级细胞壁生物合成
Shuzhen Jia1, Chunyue Wang1, Wanying Sun1
1College of Life Science, Hebei Normal University, Shijiazhuang, 050024, China.
Plant physiology and biochemistry : PPB
|June 8, 2024
概括
这项研究表明,在大米中过度表达OsWRKY12会降低干旱耐受性和二级细胞壁 (SCW) 生物合成. 这种转录因子负面调节参与SCW形成和酸 (ABA) 信号传递的基因.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 干旱压力对植物生长和作物产量产生重大影响,需要制定提高植物弹性的策略.
- 植物细胞壁在发育过程中和因环境压力而发生动态变化.
- WRKY转录因子是二次细胞壁 (SCW) 生物合成和植物对非生物应激的防御的关键调节者.
研究的目的:
- 研究OsWRKY12在大米干旱耐受性中的作用及其与二级细胞壁 (SCW) 生物合成的联系.
- 阐明OsWRKY12影响SCW形成和应激反应途径的分子机制.
主要方法:
- 定量实时PCR (qRT-PCR) 用于在干旱和酸 (ABA) 治疗下分析基因表达.
- 产生过度表达OsWRKY12的转基因大米菌株,并分析它们的耐旱性.
- 生物化学试验量化转基因和野生类型大米中的素和纤维素含量.
- 酵母单杂交 (Y1H) 和双化酶记者 (DLR) 测试以确定OsWRKY12与基因促进体的结合.
主要成果:
- 过度表达OsWRKY12降低了米在发芽和幼苗阶段的干旱耐受性.
- 与野生类型 (WT) 相比,OsWRKY12过度表达的线路显示了较低的红素和纤维素沉积量.
- OsWRKY12直接与OsABI5 (ABA信号传递) 和OsSWN3/OsSWN7 (SCW生物合成) 的促进体结合,抑制它们的表达.
结论:
- OsWRKY12对大米的干旱耐受性和SCW生物合成产生负面调节.
- 转录因子OsWRKY12作为SCW生物合成和植物应激耐受性之间的分子联系.
- OsWRKY12通过抑制ABA信号传递和SCW形成途径中的关键基因来调解这种交叉通话.
更多相关视频
相关概念视频
Responses to Drought and Flooding
10.7K
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
10.7K
Regulation of Transpiration by Stomata
28.2K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
28.2K
Adaptations that Reduce Water Loss
25.5K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.5K
Responses to Salt Stress
13.1K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.1K
Responses to Heat and Cold Stress
13.4K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.4K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K


