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电子断层扫描研究了类似虫的菌体向和网络和穿孔囊泡的演变

Wuyi Hao¹, Yuri M Chesnokov², Vyacheslav S Molchanov¹

¹Physics Department, Moscow State University, 119991 Moscow, Russia.

Journal of colloid and interface science

|June 8, 2024

概括

低温电子断层扫描 (cryo-ET) 揭示了表面活性剂聚合物结构如何从线性虫状小胞转变为穿孔囊泡. 这项研究阐明了推动这些复杂形态变化的表面活性剂自组装的机制.

科学领域:

合体和表面科学科学
材料科学材料科学材料科学
纳米技术纳米技术

背景情况:

表面活性剂自组装成微粒聚合物对于应用至关重要,但表征复杂的纳米结构是具有挑战性的.
了解体形态演变是设计基于表面活性剂的先进材料的关键.

研究的目的:

为了证明冷电子断层扫描 (cryo-ET) 对于阐明复杂的表面活性剂聚合物结构的实用性.
描述表面活性剂聚合物的结构变化作为分子包装参数的函数.

主要方法:

使用冷电子断层扫描 (cryo-ET) 与冷传输电子显微镜 (cryo-TEM),小角度中子散射 (SANS) 和风力测量一起.
研究了一种zwitterionic和 nonionic表面活性剂的模型系统,并逐渐增加 nonionic表面活性剂的摩尔分数.

主要成果:

观察到一系列的结构转变:线性虫状 (WLMs) →分支的WLMs →多连接的WLMs的和网络 →穿孔囊泡 (立体细胞).
确定转变通过增加分支和减少子链长度发生,导致立体细胞的二维膜形成.
在多连接的和网络中实验证实了指数级子链长度分布.

结论:

Cryo-ET为表面活性剂聚合物的结构演变提供了前所未有的洞察力.
已识别的结构变化的机制可以指导针对特定应用的新型表面活性剂自组件的合理设计.

关键词:

有分支的类似虫的微粒.低温电子断层扫描 (Cryo-electron tomography) 是一种电子断层扫描技术.多连接的和网络.穿孔的囊泡具有孔径.持久的长度持久的长度立体体细胞体 (Stomatosomes) 是一种细胞体.

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相关概念视频

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...