通过使用Starmerella bombicolacola进行浸泡发酵来选替代源以生产脂
Estefanía Eras-Muñoz1, Phavit Wongsirichot2, Benjamin Ingham2
1Composting Research Group (GICOM), Department of Chemical, Biological and Environmental Engineering, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Barcelona, Spain.
Waste management (New York, N.Y.)
|June 8, 2024
概括
农业副产品有效地作为生产脂的可持续源. 小麦料水解剂产生了大量的二甲基化乳C18:1,证明了其对工业生物技术的潜力.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 可持续化学 可持续化学
背景情况:
- 脂是一种多功能生物表面活性剂,具有越来越多的工业应用.
- 可持续的生产方法对于减少生物表面活性剂制造对环境的影响至关重要.
- 农业副产品是生物工艺中未充分利用的资源.
研究的目的:
- 评估农业副产品水解剂作为可持续的源用于脂生产.
- 确定最有效的解剂,以最大限度地提高脂产量和特定的同源生产.
- 为了研究水解化合物成分对索福利皮德原生基因概况的影响.
主要方法:
- 用各种农业副产品水解剂 (小麦料,大菜粉,子废物,棕废物) 作为源发酵Starmerella bombicola ATCC 22214.
- 优化总度和分析酵母菌生长和脂产生的分析.
- 索法脂原料提取物和特定的同源物 (二甲基化乳酸C18:1) 产量和生产率的表征.
- 在2-L生物反应器中进行扩大规模的研究,并使用含有疏水性碳源的小麦料化剂对同源资料的调查.
主要成果:
- 在168小时后,所有四种测试的水解剂在脂生产方面都超过了对照组.
- 小麦料和子废弃物水解剂显示了酵母生长和低于1.5g/L总的二甲酸乳C18:1产量之间的线性相关性.
- 在0.31g/L总的小麦料水解剂中,在摇瓶尺度上获得了最高的产量 (72.20g/L原料提取物,60.05g/L二甲基化乳C18:1).
- 结果在2L生物反应器中得到验证,显示二甲基化乳C18:1产量增加了15%.
- 小麦料水解剂,补充碳来源,主要产生的二甲基化乳C18:1 (88.5%重量). ),表明对同源物概况的组成影响.
结论:
- 农业副产品水解剂是可行和可持续的原料,用于脂生产.
- 小麦料水解剂是一种非常有前途的基质,可以有效地生产二甲基化乳C18:1.
- 化剂的组成显著影响了脂类同源的特征,为有针对性的生产提供了机会.
- 这项研究支持农业废物流用于生物表面活性剂制造的工业生物技术应用.
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