通过α-Ag2WO4形态依赖因素调节细胞死亡机制
Bruna D L Fragelli1, Marcelo Assis2, Joice M A Rodolpho3
1Center for Development of Functional Materials, Federal University of São Carlos (UFSCar), 13565-905 São Carlos, SP, Brazil.
概括
银酸 (α-Ag2WO4) 纳米颗粒的形态显著影响它们的细胞毒性. 纳米粒子形状影响细胞死亡路径,离子释放和反应性氧物种的产生,这对材料设计有影响.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术 纳米技术
- 生物医学工程 生物医学工程
背景情况:
- 银酸 (α-Ag2WO4) 纳米粒子正在研究潜在的生物医学应用.
- 了解纳米粒子形态和生物效应之间的关系对于安全有效的使用至关重要.
研究的目的:
- 分析具有不同形态的α-Ag2WO4的细胞毒性作用 (立方形,六角杆状,纳米杆状).
- 为了研究形态学对细胞反应在黑暗和可见光光激活下的影响.
- 阐明毒性机制,包括细胞吸收,离子释放和反应性氧/物种 (ROS/RNS) 生产.
主要方法:
- 在不同的形态上合成α-Ag2WO4:立方体 (AW-C),六角棒状 (AW-HRL) 和纳米棒状 (AW-NRL).
- 使用不同度对3T3细胞系的细胞毒性评估.
- 评估细胞吸收,离子释放,ROS和RNS的产生,以及细胞死亡途径 (亡,亡,自).
- 在没有和存在可见光光激活时效应的比较.
主要成果:
- 在所有 7.8 微克/毫升以上的形态上观察到细胞毒性.
- 负表面电荷阻止了纳米粒子的细胞内化.
- AW-NRL诱导的自,与单片氧气生成有关.
- 在光激活下,AW-HRL促进了氧化 (NO) 的产生,而AW-NRL在黑暗中没有增加.
- 光激活将细胞死亡从亡/晚期亡转移到亡和晚期亡.
结论:
- 纳米粒子形态学批判性地调节α-Ag2WO4的生物活性和毒性.
- 形态控制对于预测和优化细胞对银酸纳米颗粒的反应至关重要.
- 需要进一步的研究才能充分理解和利用这些依赖形态的效应.
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