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化剂如何防御西普罗夫洛素:从细胞内和细胞外应激反应的洞察力

Hong-Tao Shi¹, Qin-Yao Zeng¹, Xiao-Chi Feng¹

¹State Key Laboratory of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong, 518055, PR China.

|June 8, 2024

概括

抗生素,如西普罗夫洛克萨,可以抑制水中的关键脱过程. 无化细菌使用内部和外部防御,包括细胞外聚合物质 (EPS),以抵抗抗生素压力.

科学领域:

环境微生物学环境微生物学
生物地质化学循环生物地质化学循环

背景情况:

在水生环境中越来越多地检测到抗生素.
它们对微生物脱的影响和脱剂的代谢适应仍然不太清楚.

研究的目的:

调查西普洛素 (CIP) 对生物脱过程的影响.
阐明暴露于CIP的脱剂的代谢反应和防御机制.

主要方法:

通过CIP使用模型脱剂 (Paracoccus denitrificans) 对脱抑制的系统分析.
碳和代谢平衡分析.
蛋白质组学用于识别代谢途径的变化.
研究CIP效应与或没有细胞外聚合物质 (EPS).
对EPS-CIP相互作用进行光谱分析 (光和红外).

主要成果:

5 mg/L CIP显著抑制了脱化,降低了22.89%的酸盐减少,并增加了75.53%的氧化排放.
CIP破坏了电子转移和基质代谢,将脱剂转移到酸生成NADPH的酸途径,而不是NADH进行脱.
化剂的抗氧化防御不足以防止CIP诱导的氧化损伤.
细胞外聚合物 (EPS) 作为物理屏障,EPS蛋白与CIP结合并减轻其毒性作用.

结论:

脱化对抗生素污染很敏感,CIP显著损害了该过程.
无化细菌表现出细胞内代谢重编程 (例如,酸通路激活) 和细胞外防御 (EPS屏障) 来应对抗生素压力.
在保护脱剂免受抗生素引起的损害方面,EPS起着至关重要的作用.

关键词:

齐普罗夫洛克萨辛的使用方法防御战略防御战略防御战略脱化过程中的脱.细胞外聚合物物质是一种细胞外聚合物物质.基质的新陈代谢

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